Mouse anti-Human Tyrosyl-DNA Phosphodiesterase 1 Monoclonal Antibody | anti-TDP1 antibody

Tyrosyl-DNA Phosphodiesterase 1 (TDP1)

Cat. Num. AAA607047

• Gene Names: TDP1; FLJ11090; MGC104252
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: Tyrosyl-DNA Phosphodiesterase 1; Monoclonal Antibody; Tyrosyl-DNA Phosphodiesterase 1 (TDP1); Anti -Tyrosyl-DNA Phosphodiesterase 1 (TDP1); anti-TDP1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 10C312
• Specificity: Recognizes human TDP1 (1-298aa)
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied in liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-TDP1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:2000
• Immunogen: Recombinant human TDP1 (1-298aa) purified from E. coli
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-TDP1 antibody

Tyrosyl-DNA phosphodiesterase (TDP1) is a DNA repair enzyme that catalyzes the hydrolysis of a phophodiester bond between a tyrosine residue and a DNA 3' phosphate. This protein is a member of the phospholipase D (PLD) superfamily and contains two PLD phosphodiesterase domains. Mutations in TDP1 are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1) by interfering with DNA transcription or by inducting apoptosis in postmitotic neurons.

Product Categories/Family for anti-TDP1 antibody

Antibodies; Abs to Enzymes; Phosphodiesterase

NCBI and Uniprot Product Information

• NCBI GI #: 57242805
• NCBI GeneID: 55775
• NCBI Accession #: NP_001008744.1
• NCBI GenBank Nucleotide #: NM_001008744.1
• UniProt Accession #: Q9NUW8; Q2HXX4; Q96BK7; Q9NZM7; Q9NZM8; Q32Q25; Q86TV8; Q9BRS7
• Molecular Weight: 68,420 Da
• NCBI Official Full Name: tyrosyl-DNA phosphodiesterase 1
• NCBI Official Synonym Full Names: tyrosyl-DNA phosphodiesterase 1
• NCBI Official Symbol: TDP1
• NCBI Official Synonym Symbols: FLJ11090; MGC104252
• NCBI Protein Information: tyrosyl-DNA phosphodiesterase 1; tyr-DNA phosphodiesterase 1
• UniProt Protein Name: Tyrosyl-DNA phosphodiesterase 1
• Protein Family: Tyrosyl-DNA phosphodiesterase
• UniProt Gene Name: TDP1
• UniProt Entry Name: TYDP1_HUMAN

NCBI Description

The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same isoform. [provided by RefSeq]

Uniprot Description

TDP1: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Monomer. Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. Belongs to the tyrosyl-DNA phosphodiesterase family.

Protein type: Phosphodiesterase; DNA repair, damage; EC 3.1.4.-

Chromosomal Location of Human Ortholog: 14q32.11

Cellular Component: cytoplasm; nucleus

Molecular Function: tyrosyl-DNA phosphodiesterase activity; protein binding; double-stranded DNA binding; exonuclease activity; single-stranded DNA binding

Biological Process: single strand break repair; double-strand break repair; DNA repair

Disease: Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy