Mouse anti-Human, Rat Neurofilament M, 160kD Monoclonal Antibody | anti-Nefm antibody

Neurofilament M, 160kD (NF-M)

Cat. Num. AAA605999

• Gene Names: Nefm; Nfm; NF-M; Nef3; NF160; NF165
• Reactivity: Human, Rat
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Purified; Purified
• Synonyms: Neurofilament M; 160kD; Monoclonal Antibody; 160kD (NF-M); Anti -Neurofilament M; anti-Nefm antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 3G204
• Specificity: Reacts with the 160 kD polypeptides of human neurofilament. It specifically recognize a phosphateindependent epitope in the rod (core) domain of NF-M.(1,2) Reactivity with lampey NF-M has also been shown.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.

• Applicable Applications for anti-Nefm antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry, ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry.; Dilution: Flow Cytometry:; ELISA: 0.1-0.5ug/ml; Immunohistochemistry: 5-10ug/ml (paraffin-embedded or frozen sections); Immunoprecipitation: 2-5ug; Immunoblotting(1): 0.5-1.0ug/ml
• Immunogen: Rat neurofilaments
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-Nefm antibody

Neurofilament proteins (NFPs) are a macromolecular complex comprised of 3 polypeptides designated as NF-L, NF-M and NF-H. NFPs are found in the perikarya, particularly in neuronal axons throughout the central and peripheral nervous system. Since NFPs are major structural proteins and biochemically quite stable, antibodies to NFPs are useful probes in studies of neuronal expression, morphology, connectivity and pathology. The presence or absence of NFP in a variety of nervous system or neuroendocrine tumors can provide useful information about the original cell type of the tumor. In addition, the normal NFP staining pattern is altered in a variety of human diseases including Alzheimer's disease, diffuse cortical Lewy body disease, Parkinson's disease and amyotrophic lateral sclerosis (Lou Gehrig' disease). Alterations in the NFP expression pattern are also seen in most toxin-induced, sporadic and heriditary axonopathies occurring in humans and animals.

Product Categories/Family for anti-Nefm antibody

Antibodies; Abs to Neurofilament

NCBI and Uniprot Product Information

• NCBI GI #: 112363107
• NCBI GeneID: 18040
• NCBI Accession #: NP_032717.2
• NCBI GenBank Nucleotide #: NM_008691.2
• UniProt Accession #: P08553; Q0VDM8; Q3HRJ6; Q3TNS4; Q3TPK2; Q61961; Q8BQ20; A2VCT5
• Molecular Weight: 95,916 Da
• NCBI Official Full Name: neurofilament medium polypeptide
• NCBI Official Synonym Full Names: neurofilament, medium polypeptide
• NCBI Official Symbol: Nefm
• NCBI Official Synonym Symbols: Nfm; NF-M; Nef3; NF160; NF165
• NCBI Protein Information: neurofilament medium polypeptide; neurofilament-M; OTTMUSP00000017611; neurofilament 3, medium; neurofilament protein M; 160 kDa neurofilament protein; neurofilament triplet M protein
• UniProt Protein Name: Neurofilament medium polypeptide
• Protein Family: Neurofilament medium polypeptide
• UniProt Gene Name: Nefm
• UniProt Synonym Gene Names: Nef3; Nfm
• UniProt Entry Name: NFM_MOUSE

Uniprot Description

NFM: a neurofilament intermediate filament protein. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The levels of phosphorylation are altered developmentally and coincident with a change in the neurofilament function.

Protein type: Cytoskeletal

Cellular Component: neuron projection; cytoskeleton; cell; axon; neurofilament; intermediate filament; intracellular; neuromuscular junction; myelin sheath

Molecular Function: structural molecule activity

Biological Process: neurofilament cytoskeleton organization and biogenesis; axon cargo transport; regulation of axon diameter; intermediate filament cytoskeleton organization and biogenesis; intermediate filament bundle assembly; microtubule cytoskeleton organization and biogenesis