Mouse anti-Human ErbB4 Monoclonal Antibody | anti-ERBB4 antibody

ErbB4 (c-erbB4, HER4) (PE)

Cat. Num. AAA605736

• Gene Names: ERBB4; HER4; ALS19; p180erbB4
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: ErbB4; Monoclonal Antibody; ErbB4 (c-erbB4; HER4) (PE); Anti -ErbB4 (c-erbB4; anti-ERBB4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 9H77
• Specificity: Recognizes human ErbB4.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in saline, 0.5% BSA, 0.1% sodium azide. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-ERBB4 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 10ul labels up to 1x10e6 cells or 50ul of packed whole blood.
• Immunogen: Recombinant protein corresponding to aa26-649 from human ErbB4, expressed in NSO cells (Q15303).
• Preparation and Storage: May be stored at 4 degree C before opening. DO NOT FREEZE! Stable at 4 degree C as an undiluted liquid. Stable for 12 months at 4 degree C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.

Testing Data

(MCF-7 cells were stained with MBS605736 (filled histogram) or isotype control (open histogram).)

Related Product Information for anti-ERBB4 antibody

ErbB4 is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors. ErbB4 is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF). Monomeric ErbB4 binds its ligands with low affinity. Typically, heterodimerization with ErbB2 forms the high affinity receptor complex. However, ErbB4 has also been shown to heterodimerize with both ErbB1 and ErbB3. It has been suggested that the identity of the ligand may influence the dimerization partner. Several ErbB4 isoforms exist. Two of these differ in the presence of juxtamembrane extracellular sequences which regulate the ability of TACE (TNF-alpha converting enzyme, ADAM17) to proteolytically cleave ErbB4 from the cell surface. These isoforms exhibit tissue-specific expression. Another isoform lacks the phosphoinositide 3-kinase activation sequence present in the ErbB4 cytoplasmic domain. Human ErbB4 consists of 1308 amino acids (aa) with a 25aa signal sequence, a 626aa extracellular domain, a 24aa transmembrane region, and a 633aa cytoplasmic domain. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer.

Product Categories/Family for anti-ERBB4 antibody

Antibodies; Abs to Oncoproteins

NCBI and Uniprot Product Information

• NCBI GI #: 219520060
• NCBI GeneID: 2066
• UniProt Accession #: Q15303; Q2M1W1; Q59EW4; B7ZLD7; B7ZLE2; B7ZLE3
• Molecular Weight: 146,808 Da
• NCBI Official Full Name: ERBB4 protein
• NCBI Official Synonym Full Names: v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4
• NCBI Official Symbol: ERBB4
• NCBI Official Synonym Symbols: HER4; ALS19; p180erbB4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4; proto-oncogene-like protein c-ErbB-4; tyrosine kinase-type cell surface receptor HER4; v-erb-a erythroblastic leukemia viral oncogene homolog 4; avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• Protein Family: Receptor tyrosine-protein kinase
• UniProt Gene Name: ERBB4
• UniProt Synonym Gene Names: HER4; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_HUMAN

NCBI Description

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Protein kinase, TK; Protein kinase, tyrosine (receptor); Kinase, protein; Membrane protein, integral; EC 2.7.10.1; TK group; EGFR family

Chromosomal Location of Human Ortholog: 2q33.3-q34

Cellular Component: nucleoplasm; mitochondrion; mitochondrial matrix; basolateral plasma membrane; integral to membrane; plasma membrane; extracellular region; nucleus; cytosol; receptor complex

Molecular Function: protein binding; protein homodimerization activity; protein-tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity; receptor signaling protein tyrosine kinase activity; ATP binding; epidermal growth factor receptor binding

Biological Process: lactation; peptidyl-tyrosine phosphorylation; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; heart development; protein amino acid autophosphorylation; signal transduction; embryonic pattern specification; olfactory bulb interneuron differentiation; regulation of cell migration; negative regulation of cell proliferation; positive regulation of cell proliferation; neural crest cell migration; positive regulation of cardiac muscle cell proliferation; epidermal growth factor receptor signaling pathway; nervous system development; cell migration; fibroblast growth factor receptor signaling pathway; cell fate commitment; phosphoinositide-mediated signaling; transcription, DNA-dependent; positive regulation of phosphoinositide 3-kinase activity; cell proliferation; central nervous system morphogenesis; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response; positive regulation of protein amino acid phosphorylation; mitochondrial fragmentation during apoptosis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis

Disease: Amyotrophic Lateral Sclerosis 19