Mouse anti-Human Scaffold Attachment Factor B Monoclonal Antibody | anti-SAFB antibody

Scaffold Attachment Factor B (SAF-B, HET)

Cat. Num. AAA604806

• Gene Names: SAFB; HAP; HET; SAFB1; SAF-B1; DKFZp779C1727; SAFB
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Scaffold Attachment Factor B; Monoclonal Antibody; Scaffold Attachment Factor B (SAF-B; HET); Anti -Scaffold Attachment Factor B (SAF-B; anti-SAFB antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 4i59
• Specificity: Recognizes human Scaffold Attachment Factor B, Mr 130kD and an additional protein, Mr 34kD. Does not crossreact with mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.

• Applicable Applications for anti-SAFB antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in ELISA, Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Western Blot.; Dilution: Immunohistochemistry: Reported to stain SAF-B in paraf n tissue sections.; Immunocytochemistry: Reported to stain SAF-B in MCF-7 cells.; Immunoprecipitation: Reported to immunoprecipitate SAF-B from MCF-7 and HepG2 cell lysates.; Western Blot: 0.5ug/ml detects SAF-B in whole cell extracts or RIPA lysates from MCF-7 cells. MCF-7 whole cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with S0430 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse* and a chemiluminescence detection system.
• Immunogen: Synthetic peptide corresponding to aa345-357 (CPEARDKEDGRKF) from the human Scaffold Attachment Factor B conjugated to KLH (U72355). Sequence Homology: Mouse and human T-box protein 2, (8/11 identical aa).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(WB: MCF-7 whole cell lysate probed with MBS604806 (0.5ug/ml).)

Related Product Information for anti-SAFB antibody

This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis.

Product Categories/Family for anti-SAFB antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1213639
• NCBI GeneID: 6294
• UniProt Accession #: Q15424; O60406; Q59HH8; Q68DW3; A0AV56
• Molecular Weight: 102,642 Da
• NCBI Official Full Name: scaffold attachment factor B
• NCBI Official Synonym Full Names: scaffold attachment factor B
• NCBI Official Symbol: SAFB
• NCBI Official Synonym Symbols: HAP; HET; SAFB1; SAF-B1; DKFZp779C1727; SAFB
• NCBI Protein Information: scaffold attachment factor B1; SAF-B; SAB-B1; HSP27 ERE-TATA-binding protein; Hsp27 ERE-TATA binding protein; glutathione S-transferase fusion protein; HSP27 estrogen response element-TATA box-binding protein; heat-shock protein (HSP27) estrogen response element and TATA box-binding protein
• UniProt Protein Name: Scaffold attachment factor B1
• Protein Family: Scaffold attachment factor
• UniProt Gene Name: SAFB
• UniProt Synonym Gene Names: HAP; HET; SAFB1
• UniProt Entry Name: SAFB1_HUMAN

NCBI Description

This gene encodes a DNA-binding protein that has high specificity for scaffold or matrix attachment region DNA elements (S/MAR DNA). This protein is thought to be involved in attaching the base of chromatin loops to the nuclear matrix but there is conflicting evidence as to whether this protein is a component of chromatin or a nuclear matrix protein. Scaffold attachment factors are a specific subset of nuclear matrix proteins (NMP) that specifically bind to S/MAR. This encoded protein is thought to serve as a molecular base to assemble a 'transcriptosome complex' in the vicinity of actively transcribed genes. It is involved in the regulation of the heat shock protein 27 transcription and also can act as an estrogen receptor corepressor. This gene is a candidate gene for breast tumorigenesis. [provided by RefSeq]

Uniprot Description

SAFB1: Binds to scaffold/matrix attachment region (S/MAR) DNA and forms a molecular assembly point to allow the formation of a 'transcriptosomal' complex (consisting of SR proteins and RNA polymerase II) coupling transcription and RNA processing. Can function as an estrogen receptor corepressor and can also bind to the HSP27 promoter and decrease its transcription. When associated with RBMX, binds to and stimulates transcription from the SREBF1 promoter. Can inhibit cell proliferation.

Protein type: RNA-binding; Nuclear receptor co-regulator; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.3-p13.2

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; double-stranded DNA binding; nucleotide binding; chromatin binding

Biological Process: establishment and/or maintenance of chromatin architecture; estrogen receptor signaling pathway; transcription, DNA-dependent; hormone metabolic process; positive regulation of transcription from RNA polymerase II promoter; growth