Rabbit EGR1 Monoclonal Antibody | anti-egr1 antibody

EGR1 (Zif268, Early Growth Response Protein 1, Krox-24, NGFI-A, TIS8, ZENK)

Cat. Num. AAA604473

• Gene Names: egr1; krox24; krox-24; wu:fj64b05; wu:fq25f01
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: EGR1; Monoclonal Antibody; EGR1 (Zif268; Early Growth Response Protein 1; Krox-24; NGFI-A; TIS8; ZENK); Anti -EGR1 (Zif268; anti-egr1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 9j17
• Specificity: Detects endogenous levels of total EGR1. Species Crossreactivity: human, mouse and rat. Expected to cross-react with bovine due to sequence homolgy.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, and 50% glycerol.

• Applicable Applications for anti-egr1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunofluorescence, Immunocytochemistry and Immunoprecipitation.; Dilution: Western Blot: 1:1000 (Mr 75kD); Immunofluorescence (IF-IC): 1:400; Immunoprecipitation: 1:50; Flow Cytometry: 1:200
• Immunogen: Synthetic peptide derived from the amino-terminal sequence of human EGR1 (KLH coupled).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(WB analysis of extracts from serum starved PC12 cells, untreated or NGF-treated (100 ng/ml NGF for 2 hours) using MBS604473.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of PC-12 cells, untreated (blue) or NGF-treated (green), using MBS604473 compared to a non-specific negative control (red).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis analysis of dissociated PC12 cells either untreated (left) or NGF-treated for 2 hours (right), using E2215-30BC (green) and MEK1/2 (L38C12) mouse mAb (red).)

Related Product Information for anti-egr1 antibody

EGR family members are transcriptional factors with 3 repetitive Zinc finger DNA binding domains which bind to EGR response element (ER) and regulate target gene expression. The expression of EGR family members is induced by growth factors. Among the family members, EGR1 expression has been specifically shown to be induced by NGF. EGR1 expression further activates transcription of other signaling molecules including CDK5 and tyrosine hydroxylase to exert long term effects on neural cell growth and differentiation.

Zif268 is a mammalian transcription factor that is now officially known as Egr1 (Early Growth Response Protein 1). It was also named Krox-24, NGFI-A, TIS8, and ZENK. It was originally discovered in mouse.The protein encoded by this gene belongs to the EGR family of C2H2-type zinc-finger proteins. It is a nuclear protein and functions as a transcriptional regulator. The products of target genes it activates are required for differentiation and mitogenesis. Studies suggest this is a tumor suppressor gene.
The DNA binding domain of Zif268 consists of three zinc finger domains of the Cys2His2 type. The crystal structure of DNA bound by the zinc finger domain of Zif268 was solved in 1991, which greatly aided early research in zinc finger DNA-binding domains. The human Zif268/EGR1 protein contains (in its unprocessed form) 543 amino acids with a molecular weight of 57.5kD, and the gene is located on the chromosome 5.
Zif268 binds the DNA sequence 5'-GCG[G/T]GGGCG-3'. It has a distinct pattern of expression in the brain, and its induction has been shown to be associated with neuronal activity. Several studies suggest it has a role in neuronal plasticity. Zif268 has also been found to regulate the expression of synaptobrevin II (a protein important for synaptic exocytosis).
Zif268 has been shown to interact with NAB1, CEBPB, EP300, CREB binding protein, PSMA3 and P53.

Product Categories/Family for anti-egr1 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 18858601
• NCBI GeneID: 30498
• NCBI Accession #: NP_571323.1
• NCBI GenBank Nucleotide #: NM_131248.1
• UniProt Accession #: P26632
• Molecular Weight: 55,140 Da
• NCBI Official Full Name: early growth response protein 1
• NCBI Official Synonym Full Names: early growth response 1
• NCBI Official Symbol: egr1
• NCBI Official Synonym Symbols: krox24; krox-24; wu:fj64b05; wu:fq25f01
• NCBI Protein Information: early growth response protein 1; EGR-1; etID309970.14; zinc finger protein Krox-24
• UniProt Protein Name: Early growth response protein 1
• Protein Family: Early growth response protein
• UniProt Gene Name: egr1
• UniProt Synonym Gene Names: krox24
• UniProt Entry Name: EGR1_DANRE

Uniprot Description

Function: Transcriptional regulator. Recognizes and binds to the DNA sequence 5'-CGCCCCCGC-3'(EGR-site). Activates the transcription of target genes whose products are required for mitogenesis and differentiation

Subcellular location: Nucleus.

Induction: By growth factors.

Sequence similarities: Belongs to the EGR C2H2-type zinc-finger protein family.Contains 3 C2H2-type zinc fingers.