Mouse anti-Human Protein Phosphatase 2C alpha Monoclonal Antibody | anti-PPM1A antibody

Protein Phosphatase 2C alpha (PP2Ca)

Cat. Num. AAA604105

• Gene Names: PPM1A; PP2CA; MGC9201; FLJ42306; PP2Calpha; PP2C-ALPHA
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified Protein G affinity chromatography.
• Synonyms: Protein Phosphatase 2C alpha; Monoclonal Antibody; Protein Phosphatase 2C alpha (PP2Ca); Anti -Protein Phosphatase 2C alpha (PP2Ca); anti-PPM1A antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 4k14
• Specificity: Recognizes human PP2Ca (~43kD).
• Purity/Purification: Affinity Purified; Purified Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4 and 0.1% sodium azide before the addition of glycerol to 40%.

• Applicable Applications for anti-PPM1A antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:250-1:1000; Mouse liver cytosol extract was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-PP2Ca(1:250). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a DAP detection system.
• Immunogen: Recombinant human PP2Ca.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-PPM1A antibody

Protein phosphatase 2C alpha (PP2Ca) is a Mn2+- or Mg2+-dependent protein serine/threonine phosphatase that inhibits the human stress-responsive p38 and JNK MAPK pathways and regulates cellular stress response in eukaryotes. The PPM (metal-dependent protein phosphatase) family of Ser/Thr protein phosphatases have recently been shown to down-regulate the stress response pathways in eukaryotes. Within the stress pathway, key signaling kinases, which are activated by protein phosphorylation, have been proposed as the in vivo substrates of PP2C, the prototypical member of the PPM family.

Product Categories/Family for anti-PPM1A antibody

Antibodies; Abs to Enzymes; Phosphatase

NCBI and Uniprot Product Information

• NCBI GI #: 247169
• NCBI GeneID: 5494
• UniProt Accession #: P35813; O75551; B5BU11; Q5JB49
• Molecular Weight: 42,448 Da
• NCBI Official Full Name: protein phosphatase 2C alpha
• NCBI Official Synonym Full Names: protein phosphatase, Mg2+/Mn2+ dependent, 1A
• NCBI Official Symbol: PPM1A
• NCBI Official Synonym Symbols: PP2CA; MGC9201; FLJ42306; PP2Calpha; PP2C-ALPHA
• NCBI Protein Information: protein phosphatase 1A; OTTHUMP00000179040; OTTHUMP00000179041; OTTHUMP00000232007; OTTHUMP00000232008; phosphatase 2C alpha; protein phosphatase IA; protein phosphatase 2C alpha isoform; protein phosphatase 2C isoform alpha; protein phosphatase 1A (formerly 2C), magnesium-dependent, alpha isoform
• UniProt Protein Name: Protein phosphatase 1A
• Protein Family: Protein phosphatase
• UniProt Gene Name: PPM1A
• UniProt Synonym Gene Names: PPPM1A
• UniProt Entry Name: PPM1A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

Uniprot Description

PPPM1A: a Ser/Thr protein phosphatase of the PP2C family. Inhibits the activation of p38 and JNK kinase cascades induced by environmental stresses. Can also dephosphorylate cyclin-dependent kinases and may play a role in cell cycle control. Overexpression of this phosphatase activates the expression of p53, leading to G2/M cell cycle arrest and apoptosis.

Protein type: Protein phosphatase, Ser/Thr (non-receptor); EC 3.1.3.16; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 14q23.1

Cellular Component: nucleoplasm; neuron projection; membrane; voltage-gated calcium channel complex; nucleus; cytosol

Molecular Function: protein C-terminus binding; protein binding; signal transducer activity; calmodulin-dependent protein phosphatase activity; manganese ion binding; magnesium ion binding; protein serine/threonine phosphatase activity

Biological Process: N-terminal protein myristoylation; transcription initiation from RNA polymerase II promoter; Wnt receptor signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; negative regulation of I-kappaB kinase/NF-kappaB cascade; negative regulation of NF-kappaB import into nucleus; negative regulation of transcription from RNA polymerase II promoter; protein amino acid dephosphorylation; dephosphorylation; transforming growth factor beta receptor signaling pathway; insulin receptor signaling pathway; gene expression; negative regulation of transforming growth factor beta receptor signaling pathway; cell cycle arrest; positive regulation of Wnt receptor signaling pathway