Mouse anti-Human, Mouse BubR1 Monoclonal Antibody | anti-BUBR1 antibody

BubR1 (Bub1b, Bub1A, MAD3L, budding uninhibited by benzimidazoles related 1, budding uninhibited by benzimidazoles 1 homolog beta, mitotic arrest deficient-like 3 like)

Cat. Num. AAA604059

• Gene Names: BUBR1; BUB1-related (BUB1: budding uninhibited by benzymidazol 1); BUBR1; F4P9.33; F4P9_33
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: BubR1; Monoclonal Antibody; BubR1 (Bub1b; Bub1A; MAD3L; budding uninhibited by benzimidazoles related 1; budding uninhibited by benzimidazoles 1 homolog beta; mitotic arrest deficient-like 3 like); Anti -BubR1 (Bub1b; anti-BUBR1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 8J382
• Specificity: Specific for hBUBR1. There should be no crossreactivity with hBUB1. Species Crossreactivity: Human and mouse. Other species have not been tested.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.

• Applicable Applications for anti-BUBR1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.
• Immunogen: A portion of hBUBR1 tagged at the N-terminus with pMAL (residues 1-350 of human BUBR1).
• Cellular Localization: Nuclear
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-BUBR1 antibody

Compromised spindle checkpoints are thought to play an important role in genetic instability that predisposes cells to malignant transformations. Human cells contain two related checkpoint protein kinases, evolved from the BUB1 gene; hBUBR1 and hBUB1. They are important components of the spindle checkpoint Both kinases monitor mitotic kinetochore-microtubule interactions. hBUBR1 is essential for normal mitotic progression as it prevents cells from entering into anaphase too early. BUBR1 has been detected in human cancers. It has been identified as a novel binding partner of BCSG1 (Breast Cancer-Specific Gene 1), which has been suggested to accelerate the progression of breast cancer by compromising the mitotic checkpoint control through inactivation of BUBR1.

Product Categories/Family for anti-BUBR1 antibody

Antibodies; Abs to DNA Replication; Repair

NCBI and Uniprot Product Information

• NCBI GI #: 334184662
• NCBI GeneID: 817921
• NCBI Accession #: NP_001189668.1
• NCBI GenBank Nucleotide #: NM_001202739.1
• UniProt Accession #: O22806; Q8L9V1; F4IFV2
• Molecular Weight: 45,374 Da
• NCBI Official Full Name: BUB1-related protein BUBR1
• NCBI Official Symbol: BUBR1
• NCBI Official Synonym Symbols: BUB1-related (BUB1: budding uninhibited by benzymidazol 1); BUBR1; F4P9.33; F4P9_33
• NCBI Protein Information: BUB1-related protein BUBR1
• UniProt Protein Name: Mitotic spindle checkpoint protein BUBR1
• Protein Family: Mitotic spindle checkpoint protein
• UniProt Gene Name: BUBR1
• UniProt Synonym Gene Names: MAD3
• UniProt Entry Name: BUBR1_ARATH

NCBI Description

Encodes BUBR1. May have the spindle assembly checkpoint protein functions conserved from yeast to humans.

Uniprot Description

Function: Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions may be to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C

By similarity. Ref.5

Subunit structure: Interacts with anaphase-promoting complex/cyclosome (APC/C)

By similarity. Part of the mitotic checkpoint complex (MCC); interacts with CDC20-1 and CDC20-2. Interacts with MAD2 at chromocenters and with BUB3.1. Ref.5 Ref.6

Subcellular location: Chromosome. Cytoplasm. Nucleus. Chromosome › centromere › kinetochore. Cytoplasm › cytoskeleton › spindle. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome

By similarity. Note: Cytoplasmic in interphase cells. Accumulates onto both kinetochores and the spindle microtubules in cell arrested in metaphase. Associates with unattached kinetochores in early prophase upon spindle assembly checkpoint (SAC) activation. Present in chromocenters. Ref.5

Tissue specificity: Expressed in actively dividing tissues, early in organ development, in young leaves, lateral root primordia and root meristems. Ref.5

Induction: Cell cycle regulated expression with a distinct expression peak at the G2/M boundary. Ref.5

Domain: The KEN box is required for its ubiquitination and degradation

By similarity.BUB1 N-terminal domain directs kinetochore localization and binding to BUB3.

Sequence similarities: Contains 1 BUB1 N-terminal domain.