Mouse MDR2 Monoclonal Antibody | anti-ABCB4 antibody

MDR2 (MRP2, Multi Drug Resistance Associated Protein, cMOAT, Canalicular Multi Organic Anion Transporter)

Cat. Num. AAA603931

• Gene Names: ABCB4; GBD1; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunocytochemistry
• Purity: Supernatant; Supernatant
• Synonyms: MDR2; Monoclonal Antibody; MDR2 (MRP2; Multi Drug Resistance Associated Protein; cMOAT; Canalicular Multi Organic Anion Transporter); Anti -MDR2 (MRP2; anti-ABCB4 antibody

• Host: Mouse
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone Number: 6D566
• Specificity: Recognizes an internal epitope of rat MRP2. Species crossreactivity: human and mouse (Does not in react with mouse MRP2 in IHC). Does not cross-react with human P-glycoprotein, MDR3 P-glycoprotein, MRP1, MRP3 or MRP5.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid, 0.7% BSA, 0.1% sodium azide.
• Concentration: 0.25mg/m(Total protein) (varies by lot)

• Applicable Applications for anti-ABCB4 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Dilution: Immunocytochemistry: 1:20-1:50 on acetone fixed cytospin preparations.; Immunohistochemistry (frozen): 1:20 dilution on acetone fixed frozen sections followed by incubation with IgG rabbit anti-mouse IgG and a monoclonal mouse APAAP complex.; Immunohistochemistry (paraffin): Pretreatment with 0.01M citric acid, pH 6.0 in ddH2O at 100 degree C for 5 min 3 times. After washing, slides can be incubated with IgG (Biotin) rabbit anti-mouse and Streptavidin (HRP).; Note: Mouse samples are NOT suitable for use in IHC.; Western Blot: 1:20-1:50. Use an IgG (HRP) anti-mouse- as secondary antibody.
• Immunogen: Recombinant corresponding to aa1339-1541 of the C-terminal of rat MDR2.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-ABCB4 antibody

Multi-drug resistance protein 2 (MRP2), also known as cMRP, cMOAT, and ABCC2, is an ATP binding cassette (ABC) transporter and part of the multi-drug resistance (MRP) family. The MRP proteins are membrane proteins that function as organic anion pumps involved in the cellular removal of cancer drugs. MRP2 is associated with resistance to a number of cancer drugs, such as cisplatin, etoposide, doxorubicin, and methotrexate. MRP2 is predominately expressed on the apical membranes in the liver and kidney proximal tubules. It is responsible for the ATP-dependent secretion of bilirubin glucuronides and other organic anions from hepatocytes into the bile, a process important for the excretion of endogenous and xenobiotic substances. Loss of MRP2 activity is the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by defects in the secretion of anionic conjugates and the presence of melanin like pigments in hepatocytes.

Product Categories/Family for anti-ABCB4 antibody

Antibodies; Abs to Multi Drug Resistance (MDR)

NCBI and Uniprot Product Information

• NCBI GI #: 9961252
• NCBI GeneID: 5244
• NCBI Accession #: NP_061338.1
• NCBI GenBank Nucleotide #: NM_018850.2
• UniProt Accession #: O43249; P21439; Q4G0Q4; Q6PJ81; Q75MQ5
• NCBI Official Full Name: multidrug resistance protein 3 isoform C
• NCBI Official Synonym Full Names: ATP-binding cassette, sub-family B (MDR/TAP), member 4
• NCBI Official Symbol: ABCB4
• NCBI Official Synonym Symbols: GBD1; MDR2; MDR3; PGY3; ABC21; MDR2/3; PFIC-3
• NCBI Protein Information: multidrug resistance protein 3; P-glycoprotein 3; OTTHUMP00000161613; OTTHUMP00000206137; OTTHUMP00000210458; multiple drug resistance 3; ATP-binding cassette sub-family B member 4; P glycoprotein 3/multiple drug resistance 3; P-glycoprotein-3/multiple drug resistance-3
• UniProt Protein Name: Multidrug resistance protein 3
• Protein Family: ABC transporter B family
• UniProt Gene Name: MDR3
• UniProt Entry Name: O43249_HUMAN

NCBI Description

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq]

Uniprot Description

ABCB4: a transporter that mediates ATP-dependent export of organic anions and drugs from the cytoplasm. Hydrolyzes ATP with low efficiency. Human ABCB4 is not capable of conferring drug resistance. Mediates the translocation of phosphatidylcholine across the canalicular membrane of the hepatocyte. Interacts with HAX1. Defects are the cause of gallstones, and progressive familial intrahepatic cholestasis 3, an autosomal recessive liver disorder that progresses to cirrhosis and liver failure before adulthood. Two alternatively spliced human isoforms have been described.

Protein type: Membrane protein, multi-pass; EC 3.6.3.44; Transporter, ABC family; Hydrolase; Membrane protein, integral; Transporter

Chromosomal Location of Human Ortholog: 7q21.1

Cellular Component: intercellular canaliculus; membrane; integral to plasma membrane; integral to membrane; plasma membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; ATP binding; xenobiotic-transporting ATPase activity

Biological Process: response to drug; transport; multidrug transport; xenobiotic transport; lipid metabolic process; cellular lipid metabolic process; transmembrane transport

Disease: Gallbladder Disease 1; Cholestasis, Progressive Familial Intrahepatic, 3; Cholestasis, Intrahepatic, Of Pregnancy 3