Mouse anti-Hamster Nanos2 Monoclonal Antibody | anti-NANOS2 antibody

Nanos2

Cat. Num. AAA603788

• Gene Names: NANOS2; NOS2
• Reactivity: Hamster
• Applications: ELISA, Western Blot
• Purity: Purified; Purified by Protein G affinity chromatography.
• Synonyms: Nanos2; Monoclonal Antibody; Anti -Nanos2; anti-NANOS2 antibody

• Host: Mouse
• Reactivity: Hamster
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8H151
• Specificity: Recognizes human Nanos2.
• Purity/Purification: Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile 40-50% glycerol, PBS.

• Applicable Applications for anti-NANOS2 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Direct ELISA and Western Blot.; Dilution: Direct ELISA: 0.5-1.0ug/ml detection limit for rhNanos2 is ~ 2.5ng/well.; Western Blot: 1-2ug/ml detection limit for rhNanos2 is ~ 5ng/lane under non-reducing and reducing conditions.
• Immunogen: E. coli-derived rhNanos2 (aa 1 - 173)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Reconstitute to nominal volume by adding sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-NANOS2 antibody

Nanos2 is a cytoplasmic RNA-binding protein that belongs to the Nanos family of translational repressors. The 138 amino acid (aa) protein contains a 55 aa nanos-type zinc finger domain with two C2HC motifs. Human and mouse Nanos2 share identity 75% aa sequence identity overall but 95% within the zinc finger RNA binding domain. In the mouse, Nanos2 is located in male germ cells and is essential for spermatogenesis.

Product Categories/Family for anti-NANOS2 antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 71143121
• NCBI GeneID: 339345
• NCBI Accession #: NP_001025032.1
• NCBI GenBank Nucleotide #: NM_001029861.2
• UniProt Accession #: P60321; Q17R30; Q4G0P8
• Molecular Weight: 15,132 Da
• NCBI Official Full Name: nanos homolog 2
• NCBI Official Synonym Full Names: nanos homolog 2 (Drosophila)
• NCBI Official Symbol: NANOS2
• NCBI Official Synonym Symbols: NOS2
• NCBI Protein Information: nanos homolog 2; NOS-2
• UniProt Protein Name: Nanos homolog 2
• Protein Family: Nanos
• UniProt Gene Name: NANOS2
• UniProt Synonym Gene Names: NOS2
• UniProt Entry Name: NANO2_HUMAN

Uniprot Description

NANOS2: Plays a key role in the sexual differentiation of germ cells by promoting the male fate but suppressing the female fate. Represses the female fate pathways by suppressing meiosis, which in turn results in the promotion of the male fate. Maintains the suppression of meiosis by preventing STRA8 expression, which is required for premeiotic DNA replication, after CYP26B1 is decreased. Regulates the localization of the CCR4-NOT deadenylation complex to P-bodies and plays a role in recruiting the complex to trigger the degradation of mRNAs involved in meiosis. Required for the maintenance of the spermatogonial stem cell population. Not essential for the assembly of P-bodies but is required for the maintenance of their normal state. Belongs to the nanos family.

Chromosomal Location of Human Ortholog: 19q13.32

Cellular Component: perinuclear region of cytoplasm; cytoplasm; nucleus

Molecular Function: mRNA binding; protein binding; zinc ion binding

Biological Process: multicellular organismal development; negative regulation of translation; negative regulation of meiosis; mRNA catabolic process; germ-line stem cell maintenance; spermatogenesis