Mouse anti-Human Remodeling and Spacing Factor, Large Subunit Monoclonal Antibody | anti-RSF1 antibody

Remodeling and Spacing Factor, Large Subunit (RSF)

Cat. Num. AAA603727

• Gene Names: RSF1; XAP8; p325; HBXAP; RSF-1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Remodeling and Spacing Factor; Large Subunit; Monoclonal Antibody; Large Subunit (RSF); Anti -Remodeling and Spacing Factor; anti-RSF1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 5i115
• Specificity: Recognizes the large subunit (325kD) of human RSF complex. This complex is capable of facilitating ATP-dependent nucleosome remodeling and transcription initiation from chromatin templates.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Concentration: 1 mg/mL (varies by lot)

• Applicable Applications for anti-RSF1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot, and Immunoprecipitation.; Dilution: ELISA: 1:2000; Western Blot: 1:2000
• Immunogen: Purified Remodeling and Spacing Factor, RSF.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-RSF1 antibody

Transcription of naked DNA in vitro requires the general transcription factors and RNA polymerase II. However, this minimal set of factors is not sufficient for transcription when the DNA template is packaged into chromatin. Here, a factor that facilitates activator-dependent transcription initiation on chromatin templates was purified. This factor, remodeling and spacing factor (RSF), has adenosine triphosphate-dependent nucleosome-remodeling and spacing activities. Polymerases that initiate transcription with RSF can only extend their transcripts in the presence of FACT (facilitates chromatin transcription). Thus, the minimal factor requirements for activator-dependent transcription on chromatin templates in vitro have been defined.

Product Categories/Family for anti-RSF1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 251757329
• NCBI GeneID: 51773
• UniProt Accession #: Q96T23; Q86X86; Q9H3L8; Q9NVZ8; Q9NYU0; Q05DG0
• NCBI Official Full Name: Remodeling and spacing factor 1
• NCBI Official Synonym Full Names: remodeling and spacing factor 1
• NCBI Official Symbol: RSF1
• NCBI Official Synonym Symbols: XAP8; p325; HBXAP; RSF-1
• NCBI Protein Information: remodeling and spacing factor 1; OTTHUMP00000197352; OTTHUMP00000233995; HBV pX associated protein-8; HBV pX-associated protein 8; hepatitis B virus x associated protein; hepatitis B virus x-associated protein; p325 subunit of RSF chromatin-remodeling complex
• UniProt Protein Name: Remodeling and spacing factor 1
• Protein Family: Respiration factor
• UniProt Gene Name: RSF1
• UniProt Synonym Gene Names: HBXAP; XAP8
• UniProt Entry Name: RSF1_HUMAN

NCBI Description

HBXAP is involved in transcription repression, transcription coactivation when associated with hepatitis B virus X protein (HBX), and chromatin remodeling and spacing when associated with SNF2H (MIM 603375).[supplied by OMIM]

Uniprot Description

HBXAP: Required for assembly of regular nucleosome arrays by the RSF chromatin-remodeling complex. Facilitates transcription of hepatitis B virus (HBV) genes by the pX transcription activator. In case of infection by HBV, together with pX, it represses TNF- alpha induced NF-kappa-B transcription activation. Represses transcription when artificially recruited to chromatin by fusion to a heterogeneous DNA binding domain. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 11q14.1

Cellular Component: nucleoplasm; RSF complex; nucleus

Molecular Function: protein binding; zinc ion binding; histone binding; ATPase activity

Biological Process: chromatin remodeling; nucleosome assembly; DNA replication-independent nucleosome assembly at centromere; positive regulation of viral transcription; positive regulation of transcription, DNA-dependent; transcription initiation; negative regulation of transcription, DNA-dependent; nucleosome positioning; negative regulation of DNA binding