Mouse anti-Human b2-Microglobulin Monoclonal Antibody | anti-HLA-G antibody

b2-Microglobulin (B2M)

Cat. Num. AAA603726

• Gene Names: HLA-G; MHC-G
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Ascites; Ascites
• Synonyms: b2-Microglobulin; Monoclonal Antibody; b2-Microglobulin (B2M); Anti -b2-Microglobulin (B2M); anti-HLA-G antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3H36
• Specificity: Recognizes human b2-microglobulin. Species Crossreactivity: Crossreactivity is expected with mouse and rat B2M.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.

• Applicable Applications for anti-HLA-G antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:1000-1:5000 using ECL. B2M is ~12kD.; Western Blot positive control (M3890-55B) should be run to identify the appropriate B2M band and find optimum antibody dilutions.; ELISA: 1:100,000 using 50-100ng control antigen/well.
• Immunogen: Purified human B2M
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HLA-G antibody

Beta-2-microglobulin (B2M) is found in the serum of normal individuals and in the urine in elevated amounts in patients with Wilson disease, cadmium poisoning, and other conditions leading to renal tubular dysfunction. Like immunoglobulins, prealbumin, and the beta protein found in the amyloid of Alzheimer's disease, B2M has a predominantly beta-pleated sheet structure that may adopt the fibrillar configuration of amyloid in certain pathologic states. The protein is a single polypeptide chain of molecular weight 11,600D (human 119aa, chromosome 15). B2M associates with the heavy chain of class I MHC antigens on the cell surface. A transient complex of MHC-heavy chain and B2M is is known to be assembled into the TAP molecule involving interation with a number of chaperones. Binding of the processed peptide releases the class I-B2M complex to the cell surface. Absence of binding leads to degradation in the proteasome. Progressive hepatic iron overload, indistinguishable from that observed in HFE, was found only in mice homozygous for the mutated B2M gene.

Product Categories/Family for anti-HLA-G antibody

Antibodies; Abs to Serum Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 188468
• NCBI GeneID: 3135
• UniProt Accession #: O02923; O02924; O02925; O02926; O02927; O02928; O02929; O19588; O19658; O19659
• Molecular Weight: 31,549 Da
• NCBI Official Full Name: b2 microglobulin
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, G
• NCBI Official Symbol: HLA-G
• NCBI Official Synonym Symbols: MHC-G
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; OTTHUMP00000029218; OTTHUMP00000029220; OTTHUMP00000033527; OTTHUMP00000159507; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G
• UniProt Protein Name: HLA-G protein
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-G
• UniProt Entry Name: O02923_HUMAN

NCBI Description

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq]

Uniprot Description

Sequence similarities: Belongs to the MHC class I family. RuleBase RU004439