Mouse PRMT2 Monoclonal Antibody | anti-PRMT2 antibody

PRMT2 (Protein arginine N Methyltransferase 2, HRMT1L1, MGC111373)

Cat. Num. AAA603670

• Gene Names: PRMT2; HRMT1L1; MGC111373
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PRMT2; Monoclonal Antibody; PRMT2 (Protein arginine N Methyltransferase 2; HRMT1L1; MGC111373); Anti -PRMT2 (Protein arginine N Methyltransferase 2; anti-PRMT2 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8C271
• Specificity: Recognizes human PRMT2.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a sterile-filtered liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

• Applicable Applications for anti-PRMT2 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blotting.; Dilution: Western Blotting: 1:1000-1:2000
• Immunogen: Partial sequence of recombinant full-length protein to human PRMT2
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

Related Product Information for anti-PRMT2 antibody

Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology. Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.

Product Categories/Family for anti-PRMT2 antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 46255049
• NCBI GeneID: 3275
• NCBI Accession #: NP_996845.1
• NCBI GenBank Nucleotide #: NM_206962.2
• UniProt Accession #: P55345; P78350; Q6FHF0; Q9BW15; Q9UMC2; Q498Y5; Q49AF9; Q53FX4; Q5U7D4; Q99781; Q9H3L6
• Molecular Weight: 49,042 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 2 isoform 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 2
• NCBI Official Symbol: PRMT2
• NCBI Official Synonym Symbols: HRMT1L1; MGC111373
• NCBI Protein Information: protein arginine N-methyltransferase 2; PRMT2 beta; PRMT2 alpha; PRMT2 gamma; OTTHUMP00000046525; OTTHUMP00000115881; OTTHUMP00000115882; OTTHUMP00000207831; HMT1 hnRNP methyltransferase-like 1; histone-arginine N-methyltransferase PRMT2; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 1
• UniProt Protein Name: Protein arginine N-methyltransferase 2
• UniProt Gene Name: PRMT2
• UniProt Synonym Gene Names: HMT1; HRMT1L1
• UniProt Entry Name: ANM2_HUMAN

Uniprot Description

PRMT2: Arginine methyltransferase that methylates the guanidino nitrogens of arginyl residues in proteins such as STAT3, FBL, histone H4. Acts as a coactivator (with NCOA2) of the androgen receptor (AR)-mediated transactivation. Acts as a coactivator (with estrogen) of estrogen receptor (ER)-mediated transactivation. Enhances PGR, PPARG, RARA-mediated transactivation. May inhibit NF-kappa-B transcription and promote apoptosis. Represses E2F1 transcriptional activity (in a RB1- dependent manner). May be involved in growth regulation. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nucleolus; Nuclear receptor co-regulator; Methyltransferase; Methyltransferase, protein arginine; EC 2.1.1.125

Chromosomal Location of Human Ortholog: 21q22.3

Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleolus; nucleus; cytosol

Molecular Function: histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein-arginine N-methyltransferase activity; peroxisome proliferator activated receptor binding; protein homodimerization activity; retinoic acid receptor binding; transcription coactivator activity; histone-arginine N-methyltransferase activity; signal transducer activity; protein binding; androgen receptor binding; estrogen receptor binding; thyroid hormone receptor binding; progesterone receptor binding

Biological Process: histone methylation; developmental cell growth; protein amino acid methylation; inhibition of NF-kappaB transcription factor; positive regulation of apoptosis; positive regulation of transcription, DNA-dependent; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of transcription, DNA-dependent; signal transduction