Mouse Synaptopodin Monoclonal Antibody
Synaptopodin (SYNPO, KIAA1029)
Reactivity
Bovine, Guinea Pig, Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
Synaptopodin; Monoclonal Antibody; Synaptopodin (SYNPO; KIAA1029); Anti -Synaptopodin (SYNPO; anti-Synaptopodin antibody
Host
Mouse
Reactivity
Bovine, Guinea Pig, Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5F198
Specificity
Recognizes rat synaptopodin at ~44kD (degradation product). Species Crossreactivity: human, bovine, guinea pig, mouse, and gerbil. Does not recognize rabbit, frog or chicken. Recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells); co-localizes with a-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression in cultured hippocampal neurons and during postnatal maturation of rat brain.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Applicable Applications for anti-Synaptopodin antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry (frozen sections and paraffin-embedded tissue after microwave treatment): 1:5-1:10. Incubate for 1hr at RT.
Dilution: Immunohistochemistry (frozen sections and paraffin-embedded tissue after microwave treatment): 1:5-1:10. Incubate for 1hr at RT.
Immunogen
Isolated rat kidney glomeruli.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Synaptopodin antibody
Synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin, represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7kD and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74kD, pI 9.27. In SDS-PAGE the antigen appears as 100kD polypeptide in brain and 110kD polypeptide in kidney (the difference might be attributed to posttranslational modifications).
Product Categories/Family for anti-Synaptopodin antibody
NCBI and Uniprot Product Information
Uniprot Description
SYNPO: an actin-associated protein that may play a role in modulating actin-based shape and motility of dendritic spines and renal podocyte foot processes. Seems to be essential for the formation of spine apparatuses in spines of telencephalic neurons, which is involved in synaptic plasticity.
Protein type: Cytoskeletal; Actin-binding
Chromosomal Location of Human Ortholog: 5q33.1
Cellular Component: postsynaptic membrane; tight junction; postsynaptic density; cytoplasm; stress fiber; dendritic spine; perikaryon; actin cytoskeleton
Molecular Function: protein binding; actin binding
Biological Process: regulation of stress fiber formation; positive regulation of actin filament bundle formation
Similar Products
Product Notes
The Synaptopodin (Catalog #AAA603623) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Synaptopodin (SYNPO, KIAA1029) reacts with Bovine, Guinea Pig, Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Synaptopodin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry (frozen sections and paraffin-embedded tissue after microwave treatment): 1:5-1:10. Incubate for 1hr at RT. Researchers should empirically determine the suitability of the Synaptopodin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Synaptopodin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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