Mouse anti-Human Synuclein, alpha, Nitrated Monoclonal Antibody | anti-SNCA antibody

Synuclein, alpha, Nitrated

Cat. Num. AAA603584

• Gene Names: SNCA; PD1; NACP; PARK1; PARK4; MGC110988
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Synuclein; alpha; Nitrated; Monoclonal Antibody; Anti -Synuclein; anti-SNCA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 4H316
• Specificity: Recognizes the nitrated/oxidized and non-nitrated forms of human a-Synuclein protein. Cross-reactivity with b- and g-synucleins has not been observed in testing with bacterially-expressed and purified recombinant proteins. Species sequence homology
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-SNCA antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence, ELISA, Western Blot, Immunohistochemistry and Immunocytochemistry.; Dilution: Immunofluorescence (IC): 1-5ug/ml mouse; ELISA: 0.1-1.0ug/ml immunogen; Western Blot: 1-3ug/ml Band at ~18kD; Immunohistochemistry (Paraffin): 1-5ug human
• Immunogen: Recombinant full-length oxidized human alpha-Synuclein.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of WT TNM, a recombinant wild-type form of a-Synuclein that has been treated with tetranitromethane, using MBS603584)

Immunofluorescence (IF)

(Immunofluorescent staining of mouse brain (locus coeruleus) double-labeled usinf MBS603584 (red) and anti-Tyrosine Hydroxylase (green). Image courtesy of John Trojanowski, MD, PhD, University of Pennsylvania School of Medicine, PA.)

Related Product Information for anti-SNCA antibody

Alp[ha-synuclein belongs to the synuclein family, which also includes b- and g-synuclein, and is predominantly expressed in neurons, concentrated at synaptic terminals. Synucleins may be involved in the regulation of dopamine release and transport and the modulation of synaptic vesicle function. a-synuclein is a small, presynaptic protein that is the major non-amyloid component of the pathological inclusions characteristic of a wide range of neurodegenerative disorders, collectively known as synucleinopathies. Synucleinopathies include Parkinson's disease (PD), Lewy body variant of Alzheimer's disease (LBVAD), dementia with Lewy bodies (DLB), neurodegeneration with brain iron accumulation (NBIA-1), and multiple system atrophy (MSA). Although alpha-synuclein is a soluble protein, it polymerizes into filaments in vitro under a variety of conditions, lending more support to the finding that it is the major building block of the filaments that form Lewy bodies (LBs). alpha-synuclein is known to reduce the fibrillization of the microtubule-associated protein, tau, and to bind various targets such as 14-3-3, protein kinase C, synphilin-1, Elk, and Tat-binding protein 1.

Product Categories/Family for anti-SNCA antibody

Antibodies; Abs to Synuclein

NCBI and Uniprot Product Information

• NCBI GI #: 16356657
• NCBI GeneID: 6622
• UniProt Accession #: P37840; Q13701; Q4JHI3; Q6IAU6; A8K2A4; Q4W5L2; Q6QBS3
• Molecular Weight: 14,460 Da
• NCBI Official Full Name: synuclein alpha
• NCBI Official Synonym Full Names: synuclein, alpha (non A4 component of amyloid precursor)
• NCBI Official Symbol: SNCA
• NCBI Official Synonym Symbols: PD1; NACP; PARK1; PARK4; MGC110988
• NCBI Protein Information: alpha-synuclein; OTTHUMP00000161559; OTTHUMP00000161560; OTTHUMP00000161561; OTTHUMP00000218549; OTTHUMP00000218550; OTTHUMP00000218551; OTTHUMP00000218552; OTTHUMP00000218553; non-A4 component of amyloid; alpha-synuclein, isoform NACP140; non A-beta component of AD amyloid; non-A beta component of AD amyloid; Parkinson disease (autosomal dominant, Lewy body) 4
• UniProt Protein Name: Alpha-synuclein
• Protein Family: Alpha-synuclein
• UniProt Gene Name: SNCA
• UniProt Synonym Gene Names: NACP; PARK1
• UniProt Entry Name: SYUA_HUMAN

NCBI Description

Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Four alternatively spliced transcripts encoding two different isoforms have been identified for this gene. [provided by RefSeq]

Uniprot Description

SNCA: a member of the synuclein family. Abundantly expressed in the brain. Inhibits phospholipase D2 selectively. May integrate presynaptic signaling and membrane trafficking. Implicated in the pathogenesis of Parkinson's disease. A major component of amyloid plaques in the brains of patients with Alzheimer's disease. Two alternatively spliced isoforms transcripts have been identified.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: Golgi apparatus; nuclear outer membrane; mitochondrion; rough endoplasmic reticulum; lysosome; extracellular region; fibril; terminal button; cell cortex; inclusion body; cytosol; mitochondrial respiratory chain complex I; actin cytoskeleton; platelet alpha granule membrane; synaptic vesicle; growth cone; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; ribosome; nucleus; cell junction

Molecular Function: identical protein binding; protein domain specific binding; zinc ion binding; histone binding; kinesin binding; ferrous iron binding; microtubule binding; caspase inhibitor activity; beta-tubulin binding; magnesium ion binding; phosphoprotein binding; protein N-terminus binding; Hsp70 protein binding; calcium ion binding; oxidoreductase activity; dynein binding; protein binding; copper ion binding; phospholipase binding; phospholipid binding; fatty acid binding; tau protein binding; alpha-tubulin binding

Biological Process: regulation of long-term neuronal synaptic plasticity; negative regulation of serotonin uptake; regulation of acyl-CoA biosynthetic process; adult locomotory behavior; positive regulation of apoptosis; negative regulation of norepinephrine uptake; mitochondrial membrane organization and biogenesis; microglial cell activation; response to lipopolysaccharide; positive regulation of endocytosis; negative regulation of monooxygenase activity; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; dopamine biosynthetic process; fatty acid metabolic process; positive regulation of neurotransmitter secretion; regulation of dopamine secretion; negative regulation of histone acetylation; negative regulation of dopamine uptake; calcium ion homeostasis; negative regulation of exocytosis; response to magnesium ion; negative regulation of protein amino acid phosphorylation; behavioral response to cocaine; receptor internalization; phospholipid metabolic process; synapse organization and biogenesis; fibril organization and biogenesis; dopamine uptake; negative regulation of neuron apoptosis; response to iron(II) ion; positive regulation of receptor recycling; aging; caspase activation; response to drug; neutral lipid metabolic process; protein destabilization; negative regulation of microtubule polymerization; regulation of macrophage activation; regulation of glutamate secretion; positive regulation of peptidyl-serine phosphorylation; organelle ATP synthesis coupled electron transport; negative regulation of dopamine metabolic process; regulation of locomotion; synaptic vesicle endocytosis; positive regulation of release of sequestered calcium ion into cytosol; regulation of excitatory postsynaptic membrane potential; negative regulation of transporter activity; negative regulation of apoptosis

Disease: Parkinson Disease 4, Autosomal Dominant; Dementia, Lewy Body; Parkinson Disease 1, Autosomal Dominant