Immunocytochemistry (ICC)
(Figure: Immunocytochemistry usingMBS603394 GPP130 at a 1:1000 dilution.)
Mouse GPP130 Monoclonal Antibody | anti-GOLIM4 antibody
GPP130 (Golgi Phosphoprotein of 130kD, GIMPc, cis-Golgi Integral Membrane Protein)
Gene Names
GOLIM4; P138; GIMPC; GOLPH4; GPP130
Reactivity
Human, Monkey
Applications
ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedAffinity purified (HYDRA column) and gel filtrated (FPLC).
Synonyms
GPP130; Monoclonal Antibody; GPP130 (Golgi Phosphoprotein of 130kD; GIMPc; cis-Golgi Integral Membrane Protein); Anti -GPP130 (Golgi Phosphoprotein of 130kD; anti-GOLIM4 antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG1
Clone Number
6D892
Specificity
Recognizes monkey GPP130. Cross-reacts with human GPP130.
Purity/Purification
Affinity Purified
Affinity purified (HYDRA column) and gel filtrated (FPLC).
Affinity purified (HYDRA column) and gel filtrated (FPLC).
Form/Format
Lyophilized.
Applicable Applications for anti-GOLIM4 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.
Dilution: Western Blot: 1:1000.
Immunohistochemistry: Frozen sections.
Immunocytochemistry: 1:1000.
Immunoprecipitation: 2ul
Dilution: Western Blot: 1:1000.
Immunohistochemistry: Frozen sections.
Immunocytochemistry: 1:1000.
Immunoprecipitation: 2ul
Immunogen
GPP130 from Vero monkey cells.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunocytochemistry (ICC)
(Figure: Immunocytochemistry usingMBS603394 GPP130 at a 1:1000 dilution.)
Immunocytochemistry (ICC)
(Figure: Immunocytochemistry usingMBS603394 GPP130 at a 1:1000 dilution.)
Related Product Information for anti-GOLIM4 antibody
The transmembrane protein GPP130 is localized to the cis-Golgi, where it is targeted by virtue of its lumenal coiled-coil domain and mediates trafficking in the late endosome-bypass pathway. GPP130 continuously recycles between early Golgi and distal compartments.
Product Categories/Family for anti-GOLIM4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
81,880 Da
NCBI Official Full Name
Golgi integral membrane protein 4
NCBI Official Synonym Full Names
golgi integral membrane protein 4
NCBI Official Symbol
GOLIM4
NCBI Official Synonym Symbols
P138; GIMPC; GOLPH4; GPP130
NCBI Protein Information
Golgi integral membrane protein 4; golgi phosphoprotein 4; type II Golgi membrane protein; golgi phosphoprotein of 130 kDa; golgi integral membrane protein, cis; 130 kDa golgi-localized phosphoprotein; golgi-localized phosphoprotein of 130 kDa; cis Golgi-localized calcium-binding protein
UniProt Protein Name
Golgi integral membrane protein 4
Protein Family
UniProt Gene Name
GOLIM4
UniProt Synonym Gene Names
GIMPC; GOLPH4; GPP130; GIMPc
UniProt Entry Name
GOLI4_HUMAN
NCBI Description
The Golgi complex plays a key role in the sorting and modification of proteins exported from the endoplasmic reticulum. The protein encoded by this gene is a type II Golgi-resident protein. It may process proteins synthesized in the rough endoplasmic reticulum and assist in the transport of protein cargo through the Golgi apparatus. [provided by RefSeq, Jul 2008]
Uniprot Description
GOLPH4: Plays a role in endosome to Golgi protein trafficking; mediates protein transport along the late endosome-bypass pathway from the early endosome to the Golgi. Belongs to the GOLIM4 family.
Protein type: Membrane protein, integral; Vesicle
Chromosomal Location of Human Ortholog: 3q26.2
Cellular Component: nucleoplasm; Golgi apparatus; cis-Golgi network; membrane; endocytic vesicle; Golgi lumen; integral to membrane; endosome membrane
Biological Process: transport
Research Articles on GOLIM4
Similar Products
Product Notes
The GOLIM4 golim4 (Catalog #AAA603394) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The GPP130 (Golgi Phosphoprotein of 130kD, GIMPc, cis-Golgi Integral Membrane Protein) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's GPP130 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Dilution: Western Blot: 1:1000. Immunohistochemistry: Frozen sections. Immunocytochemistry: 1:1000. Immunoprecipitation: 2ul. Researchers should empirically determine the suitability of the GOLIM4 golim4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GPP130, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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