Mouse anti-Human Renin, Pro/Mature Monoclonal Antibody | anti-REN antibody

Renin, Pro/Mature

Cat. Num. AAA603354

• Gene Names: REN; HNFJ2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Renin; Pro/Mature; Monoclonal Antibody; Anti -Renin; anti-REN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7H238
• Specificity: Recognizes human Renin (pro/mature).
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS, 5% trehalose. Reconstitute with 1ml sterile ddH2O or sterile 40-50% glycerol.

• Applicable Applications for anti-REN antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in Immunoprecipitation, ELISA and Western Blot.; Dilution: Immunoprecipitation: 25ug/ml; ELISA: 0.5-1ug/ml; Western Blot: 1-2ug/ml
• Immunogen: NS0-derived recombinant human Renin pro form (aa24-406) Accession # P00797
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-REN antibody

Renin is secreted endopeptidase that plays a crucial role in the regulation of blood pressure and salt balance through the cleavage of angiotensinogen, which is the only known physiological substrate of renin. The 406 aa precursor includes a signal peptide (aa 1-23), a propeptide (aa 24-66), and a mature chain (aa 67-406). The amino acid sequence of human Renin is 100%, 73%, 71% and 67% identical to that of chimpanzee, canine, mouse and rat.

Product Categories/Family for anti-REN antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 132326
• NCBI GeneID: 5972
• UniProt Accession #: P00797; Q6FI38; Q6T5C2
• Molecular Weight: 45,057 Da
• NCBI Official Full Name: Renin
• NCBI Official Synonym Full Names: renin
• NCBI Official Symbol: REN
• NCBI Official Synonym Symbols: HNFJ2
• NCBI Protein Information: renin; angiotensinogenase; renin precursor, renal; angiotensin-forming enzyme
• UniProt Protein Name: Renin
• Protein Family: Renin
• UniProt Gene Name: REN
• UniProt Entry Name: RENI_HUMAN

NCBI Description

Renin catalyzes the first step in the activation pathway of angiotensinogen--a cascade that can result in aldosterone release,vasoconstriction, and increase in blood pressure. Renin, an aspartyl protease, cleaves angiotensinogen to form angiotensin I, which is converted to angiotensin II by angiotensin I converting enzyme, an important regulator of blood pressure and electrolyte balance. Transcript variants that encode different protein isoforms and that arise from alternative splicing and the use of alternative promoters have been described, but their full-length nature has not been determined. Mutations in this gene have been shown to cause familial hyperproreninemia. [provided by RefSeq, Jul 2008]

Uniprot Description

REN: Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. Interacts with ATP6AP2. Interaction with ATP6AP2 results in a 5-fold increased efficiency in angiotensinogen processing. Belongs to the peptidase A1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Protease; EC 3.4.23.15

Chromosomal Location of Human Ortholog: 1q32

Cellular Component: extracellular space; cytoplasm; extracellular region; plasma membrane

Molecular Function: peptidase activity; insulin-like growth factor receptor binding; aspartic-type endopeptidase activity; receptor binding

Biological Process: response to cAMP; hormone-mediated signaling; drinking behavior; male gonad development; cell maturation; renin-angiotensin regulation of aldosterone production; response to lipopolysaccharide; angiotensin maturation; proteolysis; cellular protein metabolic process; regulation of blood pressure; beta-amyloid metabolic process; regulation of MAPKKK cascade; mesonephros development; kidney development

Disease: Renal Tubular Dysgenesis; Hyperuricemic Nephropathy, Familial Juvenile, 2