Mouse anti-Equine Corticotropin Releasing Hormone Binding Protein Monoclonal Antibody | anti-CRHBP antibody

Corticotropin Releasing Hormone Binding Protein (CRHBP)

Cat. Num. AAA603129

• Gene Names: CRHBP; CRFBP; CRF-BP
• Reactivity: Equine
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Corticotropin Releasing Hormone Binding Protein; Monoclonal Antibody; Corticotropin Releasing Hormone Binding Protein (CRHBP); Anti -Corticotropin Releasing Hormone Binding Protein (CRHBP); anti-CRHBP antibody

• Host: Mouse
• Reactivity: Equine
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 7H201
• Specificity: Selected for its ability to detect human CRHBP in direct ELISAs and Western Blots. In these applications, this antibody shows no crossreactivity with rmCRHBP.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.

• Applicable Applications for anti-CRHBP antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human CRHBP. Using a colorimetric detection system, the detection limit for rhCRHBP is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human CRHBP. The detection limit for rhCRHBP is approximately 15 ng/well., Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: NS0-derived rhCRHBP
• Hybridoma: Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, NS0-derived, recombinant human Cortieotrophin-Releasing Hormone Binding Protein (rhCRHBP; aa 25-322: Accession # CAA51052).
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-CRHBP antibody

Human CRHBP is a 37kD secreted glycoprotein that likely serves to regulate CRH availability to the adrenal. In human, CRHBP complexes with CRH to form a circulating 41kD complex. In rodent and horse, this complex is restricted to the CNS. Human CRHBP is synthesized as a 322 amino acid (aa) precursor that contains a 24 aa signal sequence and a 298 aa mature region. The mature region contains one potential CUB domain and five intrachain disulfide bonds. Human mature CRHBP shares 87% and 84% sequence identity with mouse and equine CRHBP, respectively.

Product Categories/Family for anti-CRHBP antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47080099
• NCBI GeneID: 1393
• NCBI Accession #: NP_001873.2
• NCBI GenBank Nucleotide #: NM_001882.3
• UniProt Accession #: P24387; Q53F32; Q6FHT5
• Molecular Weight: 36,144 Da
• NCBI Official Full Name: corticotropin-releasing factor-binding protein
• NCBI Official Synonym Full Names: corticotropin releasing hormone binding protein
• NCBI Official Symbol: CRHBP
• NCBI Official Synonym Symbols: CRFBP; CRF-BP
• NCBI Protein Information: corticotropin-releasing factor-binding protein; CRH-BP; OTTHUMP00000128328; OTTHUMP00000222031; CRF-binding protein; corticotropin releasing hormone-binding protein; corticotropin-releasing hormone-binding protein
• UniProt Protein Name: Corticotropin-releasing factor-binding protein
• Protein Family: Corticotropin-releasing factor-binding protein
• UniProt Gene Name: CRHBP
• UniProt Synonym Gene Names: CRFBP
• UniProt Entry Name: CRHBP_HUMAN

NCBI Description

Corticotropin-releasing hormone is a potent stimulator of synthesis and secretion of preopiomelanocortin-derived peptides. Although CRH concentrations in the human peripheral circulation are normally low, they increase throughout pregnancy and fall rapidly after parturition. Maternal plasma CRH probably originates from the placenta. Human plasma contains a CRH-binding protein which inactivates CRH and which may prevent inappropriate pituitary-adrenal stimulation in pregnancy. [provided by RefSeq]

Uniprot Description

CRHBP: Binds CRF and inactivates it. May prevent inappropriate pituitary-adrenal stimulation in pregnancy. Belongs to the CRF-binding protein family.

Protein type: Inhibitor; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 5q13.3

Cellular Component: multivesicular body; Golgi apparatus; microtubule; extracellular space; endoplasmic reticulum; dendrite; perikaryon; nerve terminal; intracellular; nucleus; secretory granule

Molecular Function: protein binding; corticotropin-releasing hormone binding; peptide binding

Biological Process: negative regulation of adrenocorticotropic hormone secretion; regulated secretory pathway; synaptic transmission, dopaminergic; learning and/or memory; hormone-mediated signaling; cellular response to stress; regulation of adrenocorticotropin hormone secretion; hormone metabolic process; behavioral response to ethanol; female pregnancy; signal transduction; inflammatory response