Mouse anti-Human PAEL Receptor Monoclonal Antibody | anti-GPR37 antibody

PAEL Receptor (GPR37, G-protein Coupled Receptor 37, ETBR-LP-1, Endothelin B Receptor-like Protein 1, PAELR, Parkin-associated Endothelin Receptor-like Receptor)

Cat. Num. AAA602918

• Gene Names: GPR37; PAELR; EDNRBL; hET(B)R-LP
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: PAEL Receptor; Monoclonal Antibody; PAEL Receptor (GPR37; G-protein Coupled Receptor 37; ETBR-LP-1; Endothelin B Receptor-like Protein 1; PAELR; Parkin-associated Endothelin Receptor-like Receptor); Anti -PAEL Receptor (GPR37; anti-GPR37 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 8B38
• Specificity: Recognizes human GPR37 transfected cells but not irrelevant transfectants.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-GPR37 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: For intracellular staining to detect GPR37, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute P1799-01B to 25ug/ml and add 10ul of the diluted solution to 1-5x10e5 cells in a total reaction volume 200ul. A secondary antibody such as IgG (fluorochrome) anti-mouse can be used for visualization.
• Immunogen: NS0-derived transfected with human GPR37 (Accession #O15354)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-GPR37 antibody

GPR37 (G-protein coupled receptor 37), also called ETBR-LP-1 (endothelin B receptor-like protein 1) or PAELR (Parkin-associated endothelin receptor-like receptor) is a 613aa, ~55kD orphan 7-transmembrane receptor. It is mainly expressed in neuronal cells, particularly in cerebellar Purkinje cells and the hippocampus. It is a substrate of the E3 ubiquitin ligase, parkin, which is upregulated during endoplasmic reticulum stress. In a juvenile form of Parkinson's disease, GPR37 accumulates, contributing to stress-induced neuronal cell death. The extracellular portions of human and mouse GPR37 share 68% aa identity.

Product Categories/Family for anti-GPR37 antibody

Antibodies; Abs to G Protein Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4885323
• NCBI GeneID: 2861
• NCBI Accession #: NP_005293.1
• NCBI GenBank Nucleotide #: NM_005302.3
• UniProt Accession #: O15354; O00348; O14768; Q8TD39
• Molecular Weight: 67,114 Da
• NCBI Official Full Name: probable G-protein coupled receptor 37
• NCBI Official Synonym Full Names: G protein-coupled receptor 37 (endothelin receptor type B-like)
• NCBI Official Symbol: GPR37
• NCBI Official Synonym Symbols: PAELR; EDNRBL; hET(B)R-LP
• NCBI Protein Information: probable G-protein coupled receptor 37; ETBR-LP-1; endothelin B receptor-like protein 1; Parkin-associated endothelin receptor-like receptor
• UniProt Protein Name: Probable G-protein coupled receptor 37
• Protein Family: Prosaposin receptor
• UniProt Gene Name: GPR37
• UniProt Synonym Gene Names: ETBR-LP-1; PAELR
• UniProt Entry Name: GPR37_HUMAN

NCBI Description

This gene is a member of the G protein-coupled receptor family. The encoded protein contains seven transmembrane domains and is found in cell and endoplasmic reticulum membranes. G protein-coupled receptors are involved in translating outside signals into G protein mediated intracellular effects. This gene product interacts with Parkin and is involved in juvenile Parkinson disease. [provided by RefSeq, Oct 2012]

Uniprot Description

GPR37: Orphan receptor. May have a unique functional role in the central nervous system. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 7q31

Cellular Component: endoplasmic reticulum membrane; integral to plasma membrane; plasma membrane; ubiquitin ligase complex; receptor complex

Molecular Function: G-protein coupled receptor activity; protein binding; ubiquitin protein ligase binding; heat shock protein binding; peptide receptor activity, G-protein coupled; Hsp70 protein binding; peptide binding

Biological Process: positive regulation of dopamine metabolic process; G-protein coupled receptor protein signaling pathway; positive regulation of MAPKKK cascade; dopamine biosynthetic process; G-protein signaling, adenylate cyclase inhibiting pathway; locomotion during locomotory behavior