Flow Cytometry (FC/FACS)
(Flow cytometry using MBS601961.)
Rat anti-Mouse CD134 Ligand, Blocking Monoclonal Antibody | anti-TNFSF4 antibody
CD134 Ligand, Blocking (CD134L, OX40L, CD252)
Reactivity
Mouse
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
CD134 Ligand; Blocking; Monoclonal Antibody; Blocking (CD134L; OX40L; CD252); Anti -CD134 Ligand; anti-TNFSF4 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2c
Clone Number
6D294
Specificity
Recognizes mouse CD134L. Does not crossreact with human CD134L.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, 0.02% sodium azide, before the addition of glycerol to 40%.
Applicable Applications for anti-TNFSF4 antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Functional Assays.
Dilution: Flow Cytometry: 1ug/ml labels 5x10e5 cells.
Functional Assays: Blocks binding of CD134L to CD134.
Flow Cytometry Protocol: 1. Rat TART-gp34 cells (5x10e5) expressing surface mOX40L vector or TART-1 parental cells, were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of C2515-40.
2. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added.
3. Cells were incubated on ice for 30 min., washed and then analyzed by Flow Cytometry.
Dilution: Flow Cytometry: 1ug/ml labels 5x10e5 cells.
Functional Assays: Blocks binding of CD134L to CD134.
Flow Cytometry Protocol: 1. Rat TART-gp34 cells (5x10e5) expressing surface mOX40L vector or TART-1 parental cells, were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of C2515-40.
2. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added.
3. Cells were incubated on ice for 30 min., washed and then analyzed by Flow Cytometry.
Immunogen
Rat cells stably overexpressing mouse CD134L (OX40L).
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Flow Cytometry (FC/FACS)
(Flow cytometry using MBS601961.)
Flow Cytometry (FC/FACS)
(Flow cytometry using MBS601961.)
Product Categories/Family for anti-TNFSF4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD134(OX40)-ligand
NCBI Official Symbol
TNFSF4
NCBI Protein Information
tumor necrosis factor ligand superfamily member 4; CD134(OX40)-ligand
Protein Family
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Product Notes
The TNFSF4 (Catalog #AAA601961) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD134 Ligand, Blocking (CD134L, OX40L, CD252) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD134 Ligand, Blocking can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Functional Assays. Dilution: Flow Cytometry: 1ug/ml labels 5x10e5 cells. Functional Assays: Blocks binding of CD134L to CD134. Flow Cytometry Protocol: 1. Rat TART-gp34 cells (5x10e5) expressing surface mOX40L vector or TART-1 parental cells, were incubated on ice for 30 min. in 50ul FACS buffer (PBS, 5% fetal calf serum, 0.02% sodium azide) containing 1ug/ml of C2515-40. 2. After washing in FACS buffer, FITC-conjugated antibody to rat IgG was added. 3. Cells were incubated on ice for 30 min., washed and then analyzed by Flow Cytometry. Researchers should empirically determine the suitability of the TNFSF4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD134 Ligand, Blocking, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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