Mouse anti-Human, Mouse DNA Methyltransferase 3A Monoclonal Antibody | anti-Dnmt3a antibody

DNA Methyltransferase 3A (Dnmt 3A)

Cat. Num. AAA601364

• Gene Names: Dnmt3a; MGC109195
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified; Purified
• Synonyms: DNA Methyltransferase 3A; Monoclonal Antibody; DNA Methyltransferase 3A (Dnmt 3A); Anti -DNA Methyltransferase 3A (Dnmt 3A); anti-Dnmt3a antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 4H82
• Specificity: Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.
• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS, 0.02% sodium azide.

• Applicable Applications for anti-Dnmt3a antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 2ug/ml; Western Blot: 2ug/ml
• Immunogen: Bacteria expressed HIS-tag recombinant mouse Dnmt3a.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western Blot analysis for Dnmt3a using MBS601364 at 2ug/ml against 10ug of 293 cell lysate transfected with either mouse Dnmt3a (lane 1) or mouse Dnmt3b (lane 2).)

Related Product Information for anti-Dnmt3a antibody

Methylation of DNA at cytosine residues plays an important role in regulation of gene expression, genomic imprinting and is essential for mammalian development. Hypermethylation of CpG islands in tumor suppressor genes or hypomethylation of bulk genomic DNA may be linked with development of cancer. To date, 3 families of mammalian DNA methyltransferase genes have been identified which include Dnmt1, Dnmt2 and Dnmt3. Dnmt1 is constitutively expressed in proliferating cells and inactivation of this gene causes global demethylation of genomic DNA and embryonic lethality. Dnmt2 is expressed at low levels in adult tissues and its inactivation does not affect DNA methylation or maintenance of methylation. The Dnmt3 family members, Dnmt3a and Dnmt3b, are strongly expressed in ES cells but their expression is down regulated in differentiating ES cells and is low in adult somatic tissue. Recently, it has been shown that naturally occurring mutations of Dnmt3b gene occurs in patients with a rare autosomal recessive disorder, termed ICF (immunodeficiency, centromeric instability, and facial anomalies) syndrome.

Product Categories/Family for anti-Dnmt3a antibody

Antibodies; Abs to Enzymes; Methyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 51556265
• NCBI GeneID: 444984
• NCBI Accession #: NP_001003957.1
• NCBI GenBank Nucleotide #: NM_001003957.1
• UniProt Accession #: Q1LZ53; Q1LZ52; Q5FVL1
• Molecular Weight: 101,669 Da
• NCBI Official Full Name: DNA (cytosine-5)-methyltransferase 3A isoform 2
• NCBI Official Synonym Full Names: DNA (cytosine-5-)-methyltransferase 3 alpha
• NCBI Official Symbol: Dnmt3a
• NCBI Official Synonym Symbols: MGC109195
• NCBI Protein Information: DNA (cytosine-5)-methyltransferase 3A; DNA (cytosine-5)-methyltransferase 3A; DNA methyltransferase 3A
• UniProt Protein Name: DNA (cytosine-5)-methyltransferase 3A
• Protein Family: DNA
• UniProt Gene Name: Dnmt3a
• UniProt Entry Name: DNM3A_RAT

NCBI Description

member of a family of DNA methyltransferases [RGD]

Uniprot Description

DNMT3A: a DNA methyltransferase required for genome wide de novo methylation and essential for development. DNA methylation is coordinated with methylation of histones. Prefers CpG methylation to CpA, CpT, and CpC methylation. Methylates CpG sites at a rate much slower than DNMT1, but greater than DNMT3b. Methylation is coordinated with methylation of histones. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1 and DNMT1. Can co-localize with heterochromatin protein (HP1 ) and methyl-CpG binding protein (MeCBP). Associates with HDAC1 through its ADD-type zinc-finger. Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Protein type: Methyltransferase, DNA; Amino Acid Metabolism - cysteine and methionine; Methyltransferase; EC 2.1.1.37

Cellular Component: nucleoplasm; XY body; heterochromatin; euchromatin; nuclear matrix; cytoplasm; nuclear heterochromatin; nucleus; chromosome, pericentric region

Molecular Function: DNA (cytosine-5-)-methyltransferase activity; identical protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; unmethylated CpG binding; metal ion binding; chromatin binding

Biological Process: response to nutrient levels; response to drug; genetic imprinting; transcription, DNA-dependent; response to toxin; DNA methylation during gametogenesis; S-adenosylhomocysteine metabolic process; negative regulation of transcription from RNA polymerase II promoter; response to cocaine; response to estradiol stimulus; response to vitamin A; neuron differentiation; cytosine methylation within a CG sequence; response to ethanol; methylation-dependent chromatin silencing; response to lead ion; DNA methylation; S-adenosylmethioninamine metabolic process; spermatogenesis; response to ionizing radiation; mitotic cell cycle; DNA methylation during embryonic development; DNA methylation on cytosine; aging