Mouse anti-Human Dynamin-1-like Protein Monoclonal Antibody | anti-Dnm1l antibody

Dynamin-1-like Protein (DNM1L, Dynamin Related Protein 1, DLP1, DRP1, Dynamin-like Protein, Dnm1p/Vps1p-like Protein, DVLP, DYMPLE, DYNIV-11, FLJ41912, HDYNIV, VPS1)

Cat. Num. AAA6013527

• Gene Names: Dnm1l; DLP1; Drp1; Dnml1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Dynamin-1-like Protein; Monoclonal Antibody; Dynamin-1-like Protein (DNM1L; Dynamin Related Protein 1; DLP1; DRP1; Dynamin-like Protein; Dnm1p/Vps1p-like Protein; DVLP; DYMPLE; DYNIV-11; FLJ41912; HDYNIV; VPS1); Anti -Dynamin-1-like Protein (DNM1L; anti-Dnm1l antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,k
• Clone Number: 3B5
• Specificity: Recognizes human DNM1L.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MEALIPVINKLQDVFNTVGADIIQLPQIVVVGTQSSGKSSVLESLVGRDLLPRGTGIVTRRPLILQLVHVSQEDKRKTTGEENGVEAEEWGKFLHTKNKLYTDFDEIRQEIENETERISGNNKGVSPEPIHLKIFSPNVVNLTLVDLPGMTKVPVGDQPKDIELQIRELILRFISNPNSIILAVTAANTDMATSEALKISREVDPDGRRTLAVITKLDLMDAGTDAMDVLMGRVIPVKLGIIGVVNRSQLDINNKKSVTDSIRDEYAFLQKKYPSLANRNGTKYLARTLNRLLMHHIRDCLPELKTRINVLAAQYQSLLNSYGEPVDDKSATLLQLITKFATEYCNTIEGTAKYIETSELCGGARICYIFHETFGRTLESVDPLGGLNTIDILTAIRNATGPRPALFVPEVSFELLVKRQIKRLEEPSLRCVELVHEEMQRIIQHCSNYSTQELLRFPKLHDAIVEVVTCLLRKRLPVTNEMVHNLVAIELAYINTKHPDFADACGLMNNNIEEQRRNRLARELPSAVSRDKLIQDSRRETKNVASGGGGVGDGVQEPTTGNWRGMLKTSKAEELLAEEKSKPIPIMPASPQKGHAVNLLDVPVPVARKLSAREQRDCEVIERLIKSYFLIVRKNIQDSVPKAVMHFLVNHVKDTLQSELVGQLYKSSLLDDLLTESEDMAQRRKEAADMLKALQGASQIIAEIRETHLW

• Applicable Applications for anti-Dnm1l antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunohistochemistry.; Dilution: Immunohistochemistry (Formalin fixed paraffin embedded): 1ug/ml
• Immunogen: Full length recombinant corresponding to aa1-710 from DNM1L (AAH24590) with GST tag. MW of the GST tag alone is 26kD.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (103.84kD).)

Western Blot (WB)

(DNM1L monoclonal antibody Western Blot analysis of DNM1L expression in K-562.)

Western Blot (WB)

(Western Blot analysis of DNM1L expression in transfected 293T cell line by DNM1L monoclonal antibody.|Lane 1: DNM1L transfected lysate (79kD).|Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to DNM1L on formalin-fixed paraffin-embedded human leiomyosarcoma tissue. [antibody concentration 1ug/ml])

Testing Data

(Detection limit for recombinant GST tagged DNM1L is ~0.3ng/ml as a capture antibody.)

Related Product Information for anti-Dnm1l antibody

The protein encoded by this gene is a member of the dynamin superfamily of GTPases. Members of the dynamin-related subfamily, including the S. cerevisiae proteins Dnm1 and Vps1, contain the N-terminal tripartite GTPase domain but do not have the pleckstrin homology or proline-rich domains. This protein establishes mitochondrial morphology through a role in distributing mitochondrial tubules throughout the cytoplasm. The gene has 3 alternatively spliced transcripts encoding different isoforms. These transcripts are alternatively polyadenylated.

Product Categories/Family for anti-Dnm1l antibody

Antibodies; Abs to Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 77917614
• NCBI GeneID: 114114
• NCBI Accession #: NP_446107.2
• NCBI GenBank Nucleotide #: NM_053655.3
• UniProt Accession #: O35303; O35318; O35319; O35320; O35321; O35322; O35323; Q5U2W1; Q792T7; Q9R234; Q9R277
• Molecular Weight: 83,908 Da
• NCBI Official Full Name: dynamin-1-like protein
• NCBI Official Synonym Full Names: dynamin 1-like
• NCBI Official Symbol: Dnm1l
• NCBI Official Synonym Symbols: DLP1; Drp1; Dnml1
• NCBI Protein Information: dynamin-1-like protein; C-terminal region; N-terminal region; dynamin-like protein 1; dynamin-related protein 1
• UniProt Protein Name: Dynamin-1-like protein
• Protein Family: Dynamin-1-like protein
• UniProt Gene Name: Dnm1l
• UniProt Synonym Gene Names: Dlp1
• UniProt Entry Name: DNM1L_RAT

NCBI Description

human homolog is a GTPase that is necessary for peroxisome division [RGD, Feb 2006]

Uniprot Description

DRP1: Functions in mitochondrial and peroxisomal division. Mediates membrane fission through oligomerization into ring-like structures which wrap around the scission site to constict and sever the mitochondrial membrane through a GTP hydrolysis- dependent mechanism. Required for normal brain development. Facilitates developmentally-regulated apoptosis during neural tube development. Required for a normal rate of cytochrome c release and caspase activation during apoptosis. Also required for mitochondrial fission during mitosis. May be involved in vesicle transport. Homotetramer; dimerizes through the N-terminal GTP-middle region of one molecule binding to the GED domain of another DNM1L molecule. Can self-assemble in multimeric ring-like structures. Interacts with BCL2L1; the interaction stimulates the GTPase activity of DMN1L in synapses and increases the number of axonal mitochondria and the size and number of synaptic vesicle clusters. Interacts with FIS1. Interacts with GSK3B and MARCH5. Interacts (via the GTPase and B domains) with UBE2I; the interaction promotes sumoylation of DNM1L, mainly in ite B domain. Interacts with PPP3CA; the interaction dephosphorylates DNM1L and regulates its transition to mitochondria. Interacts witn MID49 and MID51. Ubiquitously expressed with highest levels found in skeletal muscles, heart, kidney and brain. Isoform 1 is brain-specific. Isoform 2 and isoform 3 are predominantly expressed in testis and skeletal muscles respectively. Isoform 4 is weakly expressed in brain, heart and kidney. Isoform 5 is dominantly expressed in liver, heart and kidney. Isoform 6 is expressed in neurons. Belongs to the dynamin family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.5.5; Motor; Apoptosis; Microtubule-binding; Hydrolase; Endoplasmic reticulum; Mitochondrial

Cellular Component: Golgi apparatus; microtubule; protein complex; intracellular membrane-bound organelle; mitochondrion; synaptic vesicle membrane; coated pit; cytosol; mitochondrial outer membrane; membrane; perinuclear region of cytoplasm; cytoplasm; peroxisome; cell junction

Molecular Function: GTPase activity; identical protein binding; protein binding; protein homodimerization activity; GTP binding; ubiquitin protein ligase binding; lipid binding

Biological Process: peroxisome fission; mitochondrial fission; release of cytochrome c from mitochondria; positive regulation of protein secretion; metabolic process; positive regulation of apoptosis; regulation of protein oligomerization; endocytosis; mitochondrial fragmentation during apoptosis; protein homotetramerization