Mouse S100 Calcium Binding Protein A12 Monoclonal Antibody | anti-S100A12 antibody

S100 Calcium Binding Protein A12 (S100A12, CAAF1, Calcium-binding Protein in Amniotic Fluid, Calgranulin-C, CAGC, Calgranulin Related Protein, CGRP, EN-RAGE, ENRAGE, Extracellular Newly Identified RAGE-binding Protein, Neutrophil S100 Protein, p6)

Cat. Num. AAA6013389

• Gene Names: S100A12; p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
• Applications: Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: S100 Calcium Binding Protein A12; Monoclonal Antibody; S100 Calcium Binding Protein A12 (S100A12; CAAF1; Calcium-binding Protein in Amniotic Fluid; Calgranulin-C; CAGC; Calgranulin Related Protein; CGRP; EN-RAGE; ENRAGE; Extracellular Newly Identified RAGE-binding Protein; Neutrophil S100 Protein; p6); Anti -S100 Calcium Binding Protein A12 (S100A12; anti-S100A12 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: 11n170
• Specificity: Recognizes human S100A12.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-S100A12 antibody: Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Flow Cytometry.; Dilution: Flow Cytometry: 2.5ug labels 10e6 cells
• Immunogen: Recombinant corresponding to aa1-92 from human EN-RAGE expressed in E. coli (P80511).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-S100A12 antibody

EN-RAGE, is a 10kD member of the S100 (soluble in 100% saturated ammonium sulfate) family of EF-hand calcium- binding proteins. Like other S100 proteins, S100A12 is small and generally acidic (1-3). EN-RAGE forms noncovalent homodimers in the absence of divalent cations (4-6); calcium and zinc promote the formation of higher order assemblies including tetramers and hexamers (4, 7, 8). The ability of S100A12 to chelate zinc enables it to inhibit the zinc-dependent metalloproteases MMP-2, -3, and -9 (8). S100A12 also forms heterodimers with S100A9 and binds to RAGE (Receptor for Advanced Glycation End-products), Annexin V, and several cytosolic enzymes involved in energy metabolism (9, 10). The hexameric form of EN-RAGE in particular binds RAGE with high affinity (7). EN-RAGE induces a variety of inflammatory responses including the in vivo recruitment of neutrophils, monocytes, and mast cells and the activation of mast cells and vascular endothelial cells (9,11-14). EN-RAGE is found at elevated levels under inflammatory conditions such as asthma, gout, rheumatoid arthritis synovial fluid, and atherosclerosis (8, 12, 14). S100A12 also promotes neurite outgrowth in isolated hippocampal neurons (15). An ortholog of S100A12 has not been identified in rodents, but the human protein is functional in mice and rats (11-16).

Product Categories/Family for anti-S100A12 antibody

Antibodies; Abs to Calcium Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 47124555
• NCBI GeneID: 6283
• UniProt Accession #: P80511; P83219; Q5SY66; Q7M4R1
• Molecular Weight: 10,575 Da
• NCBI Official Full Name: S100 calcium binding protein A12
• NCBI Official Synonym Full Names: S100 calcium binding protein A12
• NCBI Official Symbol: S100A12
• NCBI Official Synonym Symbols: p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE
• NCBI Protein Information: protein S100-A12; EN-RAGE; calgranulin C; calgranulin-C; neutrophil S100 protein; calcium-binding protein in amniotic fluid 1; migration inhibitory factor-related protein 6; S100 calcium-binding protein A12 (calgranulin C); extracellular newly identified RAGE-binding protein
• UniProt Protein Name: Protein S100-A12
• Protein Family: Protein
• UniProt Gene Name: S100A12
• UniProt Synonym Gene Names: CAAF1; CAGC; EN-RAGE; MRP-6; p6
• UniProt Entry Name: S10AC_HUMAN

NCBI Description

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities. [provided by RefSeq, Jul 2008]

Uniprot Description

S100A12: S100A12 is a calcium-, zinc- and copper-binding protein which plays a prominent role in the regulation of inflammatory processes and immune response. Its proinflammatory activity involves recruitment of leukocytes, promotion of cytokine and chemokine production, and regulation of leukocyte adhesion and migration. Acts as an alarmin or a danger associated molecular pattern (DAMP) molecule and stimulates innate immune cells via binding to receptor for advanced glycation endproducts (AGER). Binding to AGER activates the MAP-kinase and NF-kappa-B signaling pathways leading to production of proinflammatory cytokines and up-regulation of cell adhesion molecules ICAM1 and VCAM1. Acts as a monocyte and mast cell chemoattractant. Can stimulate mast cell degranulation and activation which generates chemokines, histamine and cytokines inducing further leukocyte recruitment to the sites of inflammation. Can inhibit the activity of matrix metalloproteinases; MMP2, MMP3 and MMP9 by chelating Zn(2+) from their active sites. Possesses filariacidal and filariastatic activity. Calcitermin possesses antifungal activity against C.albicans and is also active against E.coli and P.aeruginosa but not L.monocytogenes and S.aureus. Belongs to the S-101 family.

Chromosomal Location of Human Ortholog: 1q21

Cellular Component: cytoskeleton; cytoplasm; plasma membrane; extracellular region; cytosol; nucleus

Molecular Function: protein binding; RAGE receptor binding; copper ion binding; zinc ion binding; calcium ion binding

Biological Process: neutrophil chemotaxis; positive regulation of I-kappaB kinase/NF-kappaB cascade; killing of cells of another organism; mast cell activation; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; monocyte chemotaxis; xenobiotic metabolic process; defense response to bacterium; innate immune response; inflammatory response; defense response to fungus; cytokine secretion; positive regulation of inflammatory response