Mouse anti-Human Fucosyltransferase 3 Monoclonal Antibody | anti-FUT3 antibody

Fucosyltransferase 3 (FUT3, Galactoside 3(4)-L-fucosyltransferase, Blood Group Lewis alpha-4-Fucosyltransferase, Lewis FT)

Cat. Num. AAA601306

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Fucosyltransferase 3; Monoclonal Antibody; Fucosyltransferase 3 (FUT3; Galactoside 3(4)-L-fucosyltransferase; Blood Group Lewis alpha-4-Fucosyltransferase; Lewis FT); Anti -Fucosyltransferase 3 (FUT3; anti-FUT3 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8H187
• Specificity: Recognizes human Fucosyltransferase 3 (FUT3) and human Fucosyltransferase 5 (FUT5). Species Sequence Homology: Chimapanzee FUT5, 98%.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-FUT3 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: Suitable for use in ELISA, Western Blot and Immunoprecipitation.; Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~1ng/well.; Western Blot: 1-2ug/ml. Using a colormetric detection system, the detection limit is ~20ng/lane under non-reducing and reducing conditions.; Immunoprecipitation: 25ug/ml immunoprecipitates rhFUT3 or rhFUT5 from conditioned cell culture medium.
• Immunogen: Recombinant corresponding to aa35 -361 of human Fucosyltransferase 3 expressed in NSO cells (Accession #P21217).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-FUT3 antibody

Fucosyltransferase 3 (FUT3), also known as galactoside 3(4)-L-fucosyltransferase or blood group Lewis alpha-4-fucosyltransferase (Lewis FT), is type II membrane protein with a cytoplasmic tail (aa1-15), a transmembrane region (aa16-34) and an ectodomain (aa35-361). The amino acid sequence of human FUT3 is 99% and 52% identical to that of chimpanzee and chicken.

Product Categories/Family for anti-FUT3 antibody

Antibodies; Abs to Enzymes; Glycosyltransferase

NCBI and Uniprot Product Information

• NCBI GI #: 202957364
• UniProt Accession #: P21217; Q32NE7; Q99448; Q99449; B5U7U9; B5U7V0
• Molecular Weight: 42,117 Da
• NCBI Official Full Name: fucosyltransferase 3
• UniProt Protein Name: Galactoside 3(4)-L-fucosyltransferase
• Protein Family: Fucosyltransferase
• UniProt Gene Name: FUT3
• UniProt Synonym Gene Names: FT3B; LE
• UniProt Entry Name: FUT3_HUMAN

NCBI Description

The Lewis histo-blood group system comprises a set of fucosylated glycosphingolipids that are synthesized by exocrine epithelial cells and circulate in body fluids. The glycosphingolipids function in embryogenesis, tissue differentiation, tumor metastasis, inflammation, and bacterial adhesion. They are secondarily absorbed to red blood cells giving rise to their Lewis phenotype. This gene is a member of the fucosyltransferase family, which catalyzes the addition of fucose to precursor polysaccharides in the last step of Lewis antigen biosynthesis. It encodes an enzyme with alpha(1,3)-fucosyltransferase and alpha(1,4)-fucosyltransferase activities. Mutations in this gene are responsible for the majority of Lewis antigen-negative phenotypes. Multiple alternatively spliced variants, encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

FUT3: May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination; Lewis-positive (Le(+)) individuals have an active enzyme while Lewis-negative (Le(-)) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links. Belongs to the glycosyltransferase 10 family.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Membrane protein, integral; Transferase; EC 2.4.1.65

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: membrane; integral to membrane

Molecular Function: 3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase activity; alpha(1,3)-fucosyltransferase activity; fucosyltransferase activity

Biological Process: cell-cell recognition; biopolymer glycosylation; oligosaccharide biosynthetic process; protein amino acid glycosylation