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Western Blot (WB) (Western Blot analysis in U251, rat C6 cell line, mouse brain, rat brain tissue lysates (35ug/lane) using MBS6011658. This demonstrates MBS6011658 detected the FUBP1 protein (arrow).)

Rabbit anti-Human FUBP1 Polyclonal Antibody | anti-FUBP1 antibody

FUBP1, ID (FUBP1, Far upstream element-binding protein 1, DNA helicase V)

Gene Names
FUBP1; FBP; FUBP
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
FUBP1; Polyclonal Antibody; ID (FUBP1; Far upstream element-binding protein 1; DNA helicase V); Anti -FUBP1; anti-FUBP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Human
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-FUBP1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
FUBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 239-268 amino acids from the Central region of human FUBP1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in U251, rat C6 cell line, mouse brain, rat brain tissue lysates (35ug/lane) using MBS6011658. This demonstrates MBS6011658 detected the FUBP1 protein (arrow).)

Western Blot (WB) (Western Blot analysis in U251, rat C6 cell line, mouse brain, rat brain tissue lysates (35ug/lane) using MBS6011658. This demonstrates MBS6011658 detected the FUBP1 protein (arrow).)

Western Blot (WB)

(Western Blot analysis using MBS6011658 (1:1000) Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: U-251 MG whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 68kD. Blocking/Dilution buffer: 5% NFDM/TBST.)

Western Blot (WB) (Western Blot analysis using MBS6011658 (1:1000) Lane 1: Hela whole cell lysate Lane 2: HepG2 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: U-251 MG whole cell lysate Lysates/proteins at 20ug/lane. Secondary IgG (HRP) Goat Anti-Rabbit at 1:10,000. Predicted band size: 68kD. Blocking/Dilution buffer: 5% NFDM/TBST.)
Related Product Information for anti-FUBP1 antibody
This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase.
Product Categories/Family for anti-FUBP1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
67,560 Da
NCBI Official Full Name
far upstream element-binding protein 1
NCBI Official Synonym Full Names
far upstream element (FUSE) binding protein 1
NCBI Official Symbol
FUBP1
NCBI Official Synonym Symbols
FBP; FUBP
NCBI Protein Information
far upstream element-binding protein 1; hDH V; DNA helicase V
UniProt Protein Name
Far upstream element-binding protein 1
UniProt Gene Name
FUBP1
UniProt Synonym Gene Names
FBP; FUSE-binding protein 1; hDH V
UniProt Entry Name
FUBP1_HUMAN

NCBI Description

This gene encodes a ssDNA binding protein that activates the far upstream element (FUSE) of c-myc and stimulates expression of c-myc in undifferentiated cells. Regulation of FUSE by FUBP occurs through single-strand binding of FUBP to the non-coding strand. This protein has been shown to function as an ATP-dependent DNA helicase. [provided by RefSeq, Jul 2008]

Uniprot Description

FBP1: Regulates MYC expression by binding to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter. May act both as activator and repressor of transcription. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; RNA-binding; Transcription factor; RNA splicing; DNA-binding

Chromosomal Location of Human Ortholog: 1p31.1

Cellular Component: nucleoplasm; nucleus

Molecular Function: protein binding; transcription factor activity; single-stranded DNA binding

Biological Process: transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter

Research Articles on FUBP1

Similar Products

Product Notes

The FUBP1 fubp1 (Catalog #AAA6011658) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The FUBP1, ID (FUBP1, Far upstream element-binding protein 1, DNA helicase V) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's FUBP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the FUBP1 fubp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FUBP1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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