Dot Blot (DB)
(Dot blot analysis of mouse TSC1 Antibody (Phospho S321) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Rabbit anti-Mouse TSC1 Polyclonal Antibody
TSC1, phosphorylated (S321) (Tsc1, Kiaa0243, Hamartin, Tuberous sclerosis 1 protein homolog)
Reactivity
Mouse
Applications
ELISA, Gel Super Shift Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
TSC1; Polyclonal Antibody; phosphorylated (S321) (Tsc1; Kiaa0243; Hamartin; Tuberous sclerosis 1 protein homolog); Anti -TSC1; anti-TSC1 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-TSC1 antibody
ELISA (EL/EIA), Gel Shift Assay (GS/EMSA)
Application Notes
Suitable for use in Dot Blot, ELISA
Dilution: ELISA: 1:1,000
Dot blot 1:500
Dilution: ELISA: 1:1,000
Dot blot 1:500
Immunogen
mouse TSC1 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S321 of mouse TSC1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Dot Blot (DB)
(Dot blot analysis of mouse TSC1 Antibody (Phospho S321) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Dot Blot (DB)
(Dot blot analysis of mouse TSC1 Antibody (Phospho S321) Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)
Related Product Information for anti-TSC1 antibody
In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (By similarity).
Product Categories/Family for anti-TSC1 antibody
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a growth inhibitory protein thought to play a role in the stabilization of tuberin. Mutations in this gene have been associated with tuberous sclerosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2009]
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Product Notes
The TSC1 (Catalog #AAA6011339) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TSC1, phosphorylated (S321) (Tsc1, Kiaa0243, Hamartin, Tuberous sclerosis 1 protein homolog) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TSC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Gel Shift Assay (GS/EMSA). Suitable for use in Dot Blot, ELISA Dilution: ELISA: 1:1,000 Dot blot 1:500. Researchers should empirically determine the suitability of the TSC1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TSC1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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