Goat anti-Human OAS1 Polyclonal Antibody | anti-OAS1 antibody

Goat anti OAS1

Cat. Num. AAA226219

• Gene Names: OAS1; OIAS; IFI-4; OIASI
• Reactivity: Human
• Applications: Western Blot
• Purity: Antisera to human OAS1 were raised by repeated immunizations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Synonyms: OAS1; Polyclonal Antibody; Goat anti OAS1; OAS1 antibody; anti-OAS1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antisera to human OAS1 were raised by repeated immunizations of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Form/Format: TRIS buffered saline; 0.02% Sodium Azide; 0.5% Bovine Serum Albumin; Purified IgG - liquid
• Concentration: IgG concentration 0.5mg/ml (varies by lot)

• Applicable Applications for anti-OAS1 antibody: Western Blot (WB)
• Application Notes: WB: 1ug/ml to 3ug/ml
• Immunogen: Peptide with sequence C-THEYPHFSHRPST, from the internal region (near C terminus) of the protein sequence according to NP_058132.2
• Preparation and Storage: Store at -20 degree C only.; This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.

Western Blot (WB)

(Western blot analysis of HepG2 cell lysate (35ug protein in RIPA buffer) probed with OAS1 antibody (1ug/ml,MBS226219) and detected by chemiluminescence.)

Related Product Information for anti-OAS1 antibody

Goat anti OAS1 antibody recognizes 2'-5'-oligoadenylate synthase 1 (OAS1), also known as E18/E16 and p46/p42 OAS.

OAS1 is a member of the OAS family of genes induced by interferons (IFN) and viruses, and appears to play a role in regulation of signaling pathways during viral infection (Lee et al. 2019). The antiviral role of OAS1 appears to be attributed to its ability to detect short double stranded RNAs (dsRNAs) (Schwartz et al. 2020). In addition to viral infections, OAS proteins have been implicated in autoimmune diseases and cancer. OAS1 can affect cell migration and high OAS1 mRNA expression has been linked to worse prognosis in patients with breast cancer (Zhang and Yu 2020).

NCBI and Uniprot Product Information

• NCBI GI #: 296439492
• NCBI GeneID: 4938
• UniProt Accession #: P00973; P04820; P29080; P29081; P78485; P78486; Q16700; Q16701; Q1PG42; Q3ZM01; Q53GC5; A8K4N8
• Molecular Weight: 400
• NCBI Official Full Name: 2'-5'-oligoadenylate synthase 1
• NCBI Official Synonym Full Names: 2'-5'-oligoadenylate synthetase 1, 40/46kDa
• NCBI Official Symbol: OAS1
• NCBI Official Synonym Symbols: OIAS; IFI-4; OIASI
• NCBI Protein Information: 2'-5'-oligoadenylate synthase 1; E18/E16; p46/p42 OAS; 2-5A synthase 1; 2-5A synthetase 1; (2-5')oligo(A) synthase 1; 2',5'-oligo A synthetase 1; (2-5')oligo(A) synthetase 1; 2'-5'-oligoisoadenylate synthetase 1; 2',5'-oligoadenylate synthetase 1, 40/46kD
• UniProt Protein Name: 2'-5'-oligoadenylate synthase 1
• Protein Family: 2'-5'-oligoadenylate synthase
• UniProt Gene Name: OAS1
• UniProt Synonym Gene Names: OIAS; (2-5')oligo(A) synthase 1; 2-5A synthase 1
• UniProt Entry Name: OAS1_HUMAN

NCBI Description

This gene encodes a member of the 2-5A synthetase family, essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2',5'-oligoadenylates (2-5As). These molecules activate latent RNase L, which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Mutations in this gene have been associated with host susceptibility to viral infection. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Uniprot Description

OAS1: Interferon-induced, dsRNA-activated antiviral enzyme which plays a critical role in cellular innate antiviral response. In addition, it may also play a role in other cellular processes such as apoptosis, cell growth, differentiation and gene regulation. Synthesizes higher oligomers of 2'-5'-oligoadenylates (2-5A) from ATP which then bind to the inactive monomeric form of ribonuclease L (RNase L) leading to its dimerization and subsequent activation. Activation of RNase L leads to degradation of cellular as well as viral RNA, resulting in the inhibition of protein synthesis, thus terminating viral replication. Can mediate the antiviral effect via the classical RNase L-dependent pathway or an alternative antiviral pathway independent of RNase L. The secreted form displays antiviral effect against vesicular stomatitis virus (VSV), herpes simplex virus type 2 (HSV-2), and encephalomyocarditis virus (EMCV) and stimulates the alternative antiviral pathway independent of RNase L. Belongs to the 2-5A synthase family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transferase; EC 2.7.7.84

Chromosomal Location of Human Ortholog: 12q24.2

Cellular Component: mitochondrion; endoplasmic reticulum; cytoplasm; extracellular region; cytosol; nucleus

Molecular Function: protein binding; zinc ion binding; metal ion binding; double-stranded RNA binding; ATP binding; 2'-5'-oligoadenylate synthetase activity

Biological Process: negative regulation of viral genome replication; response to virus; cytokine and chemokine mediated signaling pathway; glucose metabolic process; purine nucleotide biosynthetic process; glucose homeostasis; defense response to virus; protein oligomerization

Disease: Diabetes Mellitus, Insulin-dependent