Rabbit anti-Human PLS1 Polyclonal Antibody | anti-PLS1 antibody

PLS1, CT (PLS1, Plastin-1, Intestine-specific plastin)

Cat. Num. AAA6011239

• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PLS1; Polyclonal Antibody; CT (PLS1; Plastin-1; Intestine-specific plastin); Anti -PLS1; anti-PLS1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PLS1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: PLS1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-579 amino acids from the C-terminal region of human PLS1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PLS1 Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the PLS1 antibody detected the PLS1 protein (arrow).)

Immunohistochemistry (IHC)

(PLS1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of PLS1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PLS1 antibody

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.

Product Categories/Family for anti-PLS1 antibody

Antibodies; Abs to Actin Related Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 21411382
• NCBI GeneID: 5357
• UniProt Accession #: Q14651; Q8NEG6; A8K2Q1; D3DNG3
• Molecular Weight: 70,253 Da
• NCBI Official Full Name: PLS1 protein
• NCBI Official Synonym Full Names: plastin 1
• NCBI Official Symbol: PLS1
• NCBI Protein Information: plastin-1; fimbrin; I plastin; intestine specific plastin; intestine-specific plastin
• UniProt Protein Name: Plastin-1
• Protein Family: Plastin
• UniProt Gene Name: PLS1
• UniProt Synonym Gene Names: I-plastin
• UniProt Entry Name: PLSI_HUMAN

NCBI Description

Plastins are a family of actin-binding proteins that are conserved throughout eukaryote evolution and expressed in most tissues of higher eukaryotes. In humans, two ubiquitous plastin isoforms (L and T) have been identified. The protein encoded by this gene is a third distinct plastin isoform, which is specifically expressed at high levels in the small intestine. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Feb 2010]

Uniprot Description

PLS1: Actin-bundling protein in the absence of calcium.

Protein type: Actin-binding; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q23

Cellular Component: cytoplasm

Molecular Function: actin filament binding; structural constituent of cytoskeleton; calcium ion binding