Rabbit anti-Human, Mouse Prkca Polyclonal Antibody | anti-PRKCA antibody

Prkca, NT (Prkca, Pkca, Protein kinase C alpha type)

Cat. Num. AAA6011047

• Gene Names: PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Prkca; Polyclonal Antibody; NT (Prkca; Pkca; Protein kinase C alpha type); Anti -Prkca; anti-PRKCA antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-PRKCA antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Flow Cytometry: 1:10-50; Immunohistochemistry: 1:10-50
• Immunogen: Mouse Prkca antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of Mouse Prkca.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Prkca Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the Prkca antibody detected the Prkca protein (arrow).)

Western Blot (WB)

(Mouse Prkca Antibody (N-term) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the Prkca antibody detected the Prkca protein (arrow).)

Flow Cytometry (FC/FACS)

(Mouse Prkca Antibody (N-term) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Immunohistochemistry (IHC)

(Mouse Prkca Antibody (N-term) (AP14277a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Prkca Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-PRKCA antibody

This is a calcium-activated, phospholipid-dependent, serine-and threonine-specific enzyme. May play a role in cell motility by phosphorylating CSPG4 (By similarity). PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.

Product Categories/Family for anti-PRKCA antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 31544941
• NCBI GeneID: 5578
• UniProt Accession #: P17252; Q15137; Q32M72; Q96RE4; B5BU22
• Molecular Weight: 76,750 Da
• NCBI Official Full Name: PRKCA protein, partial
• NCBI Official Synonym Full Names: protein kinase C, alpha
• NCBI Official Symbol: PRKCA
• NCBI Official Synonym Symbols: AAG6; PKCA; PRKACA; PKC-alpha
• NCBI Protein Information: protein kinase C alpha type; PKC-A; aging-associated gene 6
• UniProt Protein Name: Protein kinase C alpha type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCA
• UniProt Synonym Gene Names: PKCA; PRKACA; PKC-A; PKC-alpha
• UniProt Entry Name: KPCA_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.

Protein type: Kinase, protein; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.13; Oncoprotein; AGC group; PKC family; Alpha subfamily

Chromosomal Location of Human Ortholog: 17q22-q23.2

Cellular Component: nucleoplasm; photoreceptor outer segment; mitochondrion; cell soma; perinuclear region of cytoplasm; endoplasmic reticulum; apical part of cell; dendrite; mitochondrial membrane; cytoplasm; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; protein kinase C activity; enzyme binding; zinc ion binding; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; regulation of muscle contraction; mitotic nuclear envelope disassembly; positive regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of insulin receptor signaling pathway; positive regulation of mitotic cell cycle; signal transduction; protein amino acid phosphorylation; induction of positive chemotaxis; negative regulation of cell proliferation; synaptic transmission; chondrocyte differentiation; angiogenesis; cell adhesion; positive regulation of macrophage differentiation; regulation of peptidyl-tyrosine phosphorylation; regulation of the force of heart contraction; inactivation of MAPK activity; epidermal growth factor receptor signaling pathway; neutrophil chemotaxis; platelet activation; fibroblast growth factor receptor signaling pathway; positive regulation of blood vessel endothelial cell migration; adenylate cyclase activation; negative regulation of adenylate cyclase activity; cellular calcium ion homeostasis; rhodopsin mediated signaling; positive regulation of angiogenesis; negative regulation of glucose import; regulation of rhodopsin mediated signaling; phospholipase C activation; energy reserve metabolic process; innate immune response; positive regulation of endothelial cell proliferation; gene expression; mitotic cell cycle; positive regulation of protein amino acid phosphorylation; vascular endothelial growth factor receptor signaling pathway; blood coagulation; regulation of insulin secretion; positive regulation of cell migration; positive regulation of inflammatory response