Rabbit anti-Human CCNT1 Polyclonal Antibody | anti-CCNT1 antibody

CCNT1, ID (Cyclin T1, Cyclin-T1, CycT1, Cyclin-T)

Cat. Num. AAA6010501

• Gene Names: CCNT1; CCNT; CYCT1; HIVE1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry
• Purity: Serum; Serum
• Synonyms: CCNT1; Polyclonal Antibody; ID (Cyclin T1; Cyclin-T1; CycT1; Cyclin-T); Anti -CCNT1; anti-CCNT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CCNT1.
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in 0.02M potassium phosphate, 0.15M sodium chloride, pH 7.2, 0.01% sodium azide.

• Applicable Applications for anti-CCNT1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry.; Dilution: ELISA: 1:1000-1:5000; Western Blot: 1:500-1:2000; Immunohistochemistry (Formalin fixed paraffin embedded): 1:500
• Immunogen: Synthetic peptide corresponding to the internal region of human CCNT1 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Skin (formalin-fixed, paraffin-embedded) stained with CCNT1 antibody at 1:500 followed by biotinylated goat rabbit IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Related Product Information for anti-CCNT1 antibody

Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes.

Product Categories/Family for anti-CCNT1 antibody

Antibodies; Abs to Cyclin

NCBI and Uniprot Product Information

• NCBI GI #: 482661631
• NCBI GeneID: 904
• NCBI Accession #: NP_001264771.1
• NCBI GenBank Nucleotide #: NM_001277842.1
• UniProt Accession #: O60563; O60581
• Molecular Weight: 80,685 Da
• NCBI Official Full Name: cyclin-T1 isoform b
• NCBI Official Synonym Full Names: cyclin T1
• NCBI Official Symbol: CCNT1
• NCBI Official Synonym Symbols: CCNT; CYCT1; HIVE1
• NCBI Protein Information: cyclin-T1; cyclin C-related protein; CDK9-associated C-type protein; human immunodeficiency virus type 1 (HIV-1) expression (elevated) 1
• UniProt Protein Name: Cyclin-T1
• Protein Family: Cyclin
• UniProt Gene Name: CCNT1
• UniProt Synonym Gene Names: CycT1; Cyclin-T
• UniProt Entry Name: CCNT1_HUMAN

NCBI Description

This gene encodes a member of the highly conserved cyclin C subfamily. The encoded protein tightly associates with cyclin-dependent kinase 9, and is a major subunit of positive transcription elongation factor b (p-TEFb). In humans, there are multiple forms of positive transcription elongation factor b, which may include one of several different cyclins along with cyclin-dependent kinase 9. The complex containing the encoded cyclin and cyclin-dependent kinase 9 acts as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and is both necessary and sufficient for full activation of viral transcription. This cyclin and its kinase partner are also involved in triggering transcript elongation through phosphorylation of the carboxy-terminal domain of the largest RNA polymerase II subunit. Overexpression of this gene is implicated in tumor growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

CCNT1: Regulatory subunit of the cyclin-dependent kinase pair (CDK9/cyclin-T1) complex, also called positive transcription elongation factor B (P-TEFb), which is proposed to facilitate the transition from abortive to productive elongation by phosphorylating the CTD (carboxy-terminal domain) of the large subunit of RNA polymerase II (RNA Pol II). In case of HIV or SIV infections, binds to the transactivation domain of the viral nuclear transcriptional activator, Tat, thereby increasing Tat's affinity for the transactivating response RNA element (TAR RNA). Serves as an essential cofactor for Tat, by promoting RNA Pol II activation, allowing transcription of viral genes. Cyclin-T1 is the predominant cyclin that associates with CDK9 to form a heterodimer called P-TEFb. P-TEFb forms a complex with AFF4/AF5Q31. Interacts with the transactivation region of HIV-1, HIV-2 and SIV Tat. Component of a complex which is at least composed of HTATSF1/Tat-SF1, P-TEFb complex, RNA pol II, SUPT5H, and NCL/nucleolin. Component of the 7SK snRNP complex at least composed of P-TEFb (composed of CDK9 and CCNT1/cyclin-T1), HEXIM1, HEXIM2, BCDIN3, SART3 proteins and 7SK and U6 snRNAs. Interacts with MDFIC. Ubiquitously expressed. Belongs to the cyclin family. Cyclin C subfamily.

Protein type: Cell cycle regulation; Transcription initiation complex

Chromosomal Location of Human Ortholog: 12q13.11

Cellular Component: nucleoplasm; transcription elongation factor complex b; nucleolus; nucleus

Molecular Function: protein binding; snRNA binding; DNA binding; chromatin binding; protein kinase binding

Biological Process: transcription from RNA polymerase II promoter; transcription initiation from RNA polymerase II promoter; viral reproduction; transcription, DNA-dependent; positive regulation of viral transcription; cell cycle; protein amino acid phosphorylation; cell division; transforming growth factor beta receptor signaling pathway; RNA elongation from RNA polymerase II promoter; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of cyclin-dependent protein kinase activity