Mouse anti-Rat Glutamate Decarboxylase 65 Monoclonal Antibody | anti-Gad2 antibody
Glutamate Decarboxylase 65 (GAD65)
Gene Names
Gad2; GAD65; Gad-2; GAD(65); 6330404F12Rik
Reactivity
Rat
Applications
ELISA, Western Blot, Immunoprecipitation
Purity
AscitesAscites.
Synonyms
Glutamate Decarboxylase 65; Monoclonal Antibody; Glutamate Decarboxylase 65 (GAD65); Anti -Glutamate Decarboxylase 65 (GAD65); anti-Gad2 antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3H23
Specificity
Recognizes rat Glutamate Decarboxylase 65 (GAD65). Species Seqeunce Homology
Purity/Purification
Ascites
Ascites.
Ascites.
Form/Format
Supplied as a liquid in PBS, 0.05% sodium azide, 40% glycerol.
Applicable Applications for anti-Gad2 antibody
ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP)
Application Notes
Suitable for use in ELISA, Immunoprecipitation and Western Blot.
Dilution: Western Blot: 1:1000- 1:5000. ~65kD band in rat brain.
ELISA: 1:10,000-1:100,000 using 50-100ng UCP2 control peptide/well.
Immunoprecipitation: 10ul.
Dilution: Western Blot: 1:1000- 1:5000. ~65kD band in rat brain.
ELISA: 1:10,000-1:100,000 using 50-100ng UCP2 control peptide/well.
Immunoprecipitation: 10ul.
Immunogen
An 18aa peptide sequence near the N-terminus of rat GAD65.
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-Gad2 antibody
Gamma-Aminobutyric acid (GABA) is the major inhibitory neurotransmitter in the brain. Glutamic acid decarboxylase (GAD; E.C.4.1.1.15) catalyzes the decarboxylation of glutamate to produce GABA-the rate limiting step in the synthesis of GABA. The two major isoform of GAD, GAD67 (MW 67kD) and GAD65 (MW 65kD) are derived from distinct genes and differ in their subcellular distribution. GAD67 is 594 aa single chain polypeptide encoded by human chromosome 2 gene, whereas GAD65 is am amphiphilic and membrane anchored protein (585 aa, chromosome 10). GAD isoform shares ~65% homology. GAD is also a major autoantigen associated with SMS (stiff-man syndrome).
Product Categories/Family for anti-Gad2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
1,989 Da
NCBI Official Full Name
glutamate decarboxylase 65
NCBI Official Synonym Full Names
glutamic acid decarboxylase 2
NCBI Official Symbol
Gad2
NCBI Official Synonym Symbols
GAD65; Gad-2; GAD(65); 6330404F12Rik
NCBI Protein Information
glutamate decarboxylase 2; GAD-65; OTTMUSP00000012268; 65 kDa glutamic acid decarboxylase; glutamate decarboxylase 65 kDa isoform
UniProt Protein Name
Glutamate decarboxylase 65
Protein Family
UniProt Gene Name
Gad2
UniProt Synonym Gene Names
gad65
UniProt Entry Name
Q9EQX8_MOUSE
Uniprot Description
GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.
Protein type: Carbohydrate Metabolism - butanoate; Other Amino Acids Metabolism - taurine and hypotaurine; EC 4.1.1.15; Other Amino Acids Metabolism - beta-alanine; Lyase; Amino Acid Metabolism - alanine, aspartate and glutamate
Cellular Component: Golgi apparatus; synaptic vesicle membrane; membrane; axon; perinuclear region of cytoplasm; cytoplasm; plasma membrane; synapse; cytoplasmic vesicle; cytosol; cell junction
Molecular Function: glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; lyase activity; carboxy-lyase activity; catalytic activity; pyridoxal phosphate binding
Biological Process: glutamate decarboxylation to succinate; carboxylic acid metabolic process; neurotransmitter biosynthetic process
Research Articles on Gad2
Similar Products
Product Notes
The Gad2 gad2 (Catalog #AAA601045) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Glutamate Decarboxylase 65 (GAD65) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamate Decarboxylase 65 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunoprecipitation (IP). Suitable for use in ELISA, Immunoprecipitation and Western Blot. Dilution: Western Blot: 1:1000- 1:5000. ~65kD band in rat brain. ELISA: 1:10,000-1:100,000 using 50-100ng UCP2 control peptide/well. Immunoprecipitation: 10ul. Researchers should empirically determine the suitability of the Gad2 gad2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamate Decarboxylase 65, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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