Mouse anti-Human HLA Class 1 Antigen B17,B35,B44(X-Reactive) Monoclonal Antibody
HLA Class 1 Antigen B17,B35,B44(X-Reactive)
Reactivity
Human
Applications
Microcytotoxicity Test
Purity
Ascites
Synonyms
HLA Class 1 Antigen B17; B35; B44(X-Reactive); Monoclonal Antibody; Anti -HLA Class 1 Antigen B17; anti-HLA Class 1 Antigen B17; B44(X-Reactive) antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgM
Clone Number
8.L.230
Specificity
Recognizes human HLA Class 1Antigen-B17,B35,B44. Additional cross reactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH.
Purity/Purification
Ascites
Form/Format
Supplied as a lyophilized powder. Reconstitute with 100ul sterile ddH2O to make a 10X stock solution.
Applicable Applications for anti-HLA Class 1 Antigen B17,B35,B44(X-Reactive) antibody
Microcytotoxicity Test
Application Notes
This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies.
Directions for Use
For a cytotoxic working dilution, add 0.9ml of 1% BSA in PBS to 100ul of10X working stock. Store aliquots at -20°C.
Expected Values
Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.
Limitations
Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
Preparation and Storage
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-HLA Class 1 Antigen B17,B35,B44(X-Reactive) antibody
HLA CLass I is involved in the presentation of foreign antigens to the immune system. This antibody is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders.
Product Categories/Family for anti-HLA Class 1 Antigen B17,B35,B44(X-Reactive) antibody
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Product Notes
The HLA Class 1 Antigen B17,B35,B44(X-Reactive) (Catalog #AAA601015) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The HLA Class 1 Antigen B17,B35,B44(X-Reactive) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's HLA Class 1 Antigen B17,B35,B44(X-Reactive) can be used in a range of immunoassay formats including, but not limited to, Microcytotoxicity Test. This antibody has not been tested in methodologies other than Microcytotoxicity Test. Potential applications include Flow Cytometry, Cell Typing, Tissue Staining and Chimerism Studies. Researchers should empirically determine the suitability of the HLA Class 1 Antigen B17,B35,B44(X-Reactive) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HLA Class 1 Antigen B17,B35,B44(X-Reactive), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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