Mouse anti-Human HLA Class 1 Antigen A25,A32 Monoclonal Antibody

HLA Class 1 Antigen A25,A32

Cat. Num. AAA602885

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Ascites; Ascites
• Synonyms: HLA Class 1 Antigen A25; A32; Monoclonal Antibody; Anti -HLA Class 1 Antigen A25; anti-HLA Class 1 Antigen A25; A32 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgM
• Clone Number: 4i99
• Specificity: Reacts to HLA Class 1 Antigen-A25,A32. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
• Purity/Purification: Ascites; Ascites
• Form/Format: Supplied as a lyophilized powder in PBS, 1% BSA. Reconstitute to 100ul sterile dH2O to make a 10X stock solution.

• Applicable Applications for anti-HLA Class 1 Antigen A25,A32 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Flow Cytometry, Cell Typing, Tissue Staining, and Chimerism Studies.; Dilution: Cytotoxicity: Add 0.9ml of 1% BSA in PBS to 100ul of10X working dilution.
• Immunogen: HLA Class 1 Antigen-A25, A32.
• Cytotoxicity: Expected Results:; Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction.; Limitations:; Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.; Please contact MyBioSource for bulk requirements and licensing requests relating to production applications.

Testing Data

Related Product Information for anti-HLA Class 1 Antigen A25,A32 antibody

The human leukocyte antigen system (HLA) is the name of the major histocompatibility complex (MHC) in humans. The superlocus contains a large number of genes related to immune system function in humans. This group of genes resides on chromosome 6, and encodes cell-surface antigen-presenting proteins and many other genes. The HLA genes are the human versions of the MHC genes that are found in most vertebrates (and thus are the most studied of the MHC genes). The proteins encoded by certain genes are also known as antigens, as a result of their historic discovery as factors in organ transplantations. The major HLA antigens are essential elements for immune function. Different classes have different functions: HLA class I antigens (A, B & C) present peptides from inside the cell (including viral peptides if present). These peptides are produced from digested proteins that are broken down in the proteasomes. The peptides are generally small polymers, about 9 amino acids in length. Foreign antigens attract killer T-cells (also called CD8 positive- or cytotoxic T-cells) that destroy cells.

HLA class II antigens (DP,DM, DOA,DOB,DQ, & DR) present antigens from outside of the cell to T-lymphocytes. These particular antigens stimulate T-helper cells to multiply, and these T-helper cells then stimulate antibody-producing B-cells to produce Antibodies to that specific antigen. Self-antigens are suppressed by suppressor T-cells.

Product Categories/Family for anti-HLA Class 1 Antigen A25,A32 antibody

Antibodies; Abs to HLA