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Western Blot (WB) (Western Blot analysis for KRTHB1 expression in HepG2 |using MBS6009592 .)

Mouse anti-Human KRT81 Monoclonal Antibody | anti-KRT81 antibody

KRT81 (Keratin, Type II Cuticular Hb1, Hair Keratin K2.9, Keratin, Hair, Basic, 1, Keratin-81, K81, Metastatic Lymph Node 137 Gene Protein, MLN 137, Type II Hair Keratin Hb1, Type-II Keratin Kb21, ghHKb1, ghHb1, KRTHB1, MLN137)

Gene Names
KRT81; HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1
Reactivity
Human
Applications
ELISA, Western Blot, Immunohistochemistry, Immunofluorescence
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
KRT81; Monoclonal Antibody; KRT81 (Keratin; Type II Cuticular Hb1; Hair Keratin K2.9; Keratin; Hair; Basic; 1; Keratin-81; K81; Metastatic Lymph Node 137 Gene Protein; MLN 137; Type II Hair Keratin Hb1; Type-II Keratin Kb21; ghHKb1; ghHb1; KRTHB1; MLN137); Anti -KRT81 (Keratin; anti-KRT81 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
3B10-5B10
Specificity
Recognizes human KRTHB1.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2.
Applicable Applications for anti-KRT81 antibody
ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Dilution: Immunofluorescence: 10ug/ml
Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml
Immunogen
Recombinant corresponding to aa1-203 from human KRTHB1 with GST tag. MW of the GST tag alone is 26kD.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis for KRTHB1 expression in HepG2 |using MBS6009592 .)

Western Blot (WB) (Western Blot analysis for KRTHB1 expression in HepG2 |using MBS6009592 .)

Immunofluorescence (IF)

(Immunofluorescence of HeLa cell using MBS6009592 (10ug/ml))

Immunofluorescence (IF) (Immunofluorescence of HeLa cell using MBS6009592 (10ug/ml))

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of FFPE human prostate after HIER using sodium citrate buffer pH6.0 using MBS6009592 (5ug/ml).)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of FFPE human prostate after HIER using sodium citrate buffer pH6.0 using MBS6009592 (5ug/ml).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of FFPE human skin after HIER using sodium citrate buffer pH6.0 using MBS6009592 (5ug/ml).)

Immunohistochemistry (IHC) (Immunohistochemistry analysis of FFPE human skin after HIER using sodium citrate buffer pH6.0 using MBS6009592 (5ug/ml).)
Related Product Information for anti-KRT81 antibody
The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq]
Product Categories/Family for anti-KRT81 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
54,928 Da
NCBI Official Full Name
keratin, type II cuticular Hb1
NCBI Official Synonym Full Names
keratin 81
NCBI Official Symbol
KRT81
NCBI Official Synonym Symbols
HB1; Hb-1; KRTHB1; MLN137; ghHkb1; hHAKB2-1
NCBI Protein Information
keratin, type II cuticular Hb1; K81; ghHb1; MLN 137; keratin-81; hair keratin K2.9; type-II keratin Kb21; keratin, hair, basic, 1; hard keratin, type II, 1; type II hair keratin Hb1; metastatic lymph node 137 gene protein
UniProt Protein Name
Keratin, type II cuticular Hb1
Protein Family
UniProt Gene Name
KRT81
UniProt Synonym Gene Names
KRTHB1; MLN137; K81; MLN 137; ghHb1
UniProt Entry Name
KRT81_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. As a type II hair keratin, it is a basic protein which heterodimerizes with type I keratins to form hair and nails. The type II hair keratins are clustered in a region of chromosome 12q13 and are grouped into two distinct subfamilies based on structure similarity. One subfamily, consisting of KRTHB1, KRTHB3, and KRTHB6, is highly related. The other less-related subfamily includes KRTHB2, KRTHB4, and KRTHB5. All hair keratins are expressed in the hair follicle; this hair keratin, as well as KRTHB3 and KRTHB6, is found primarily in the hair cortex. Mutations in this gene and KRTHB6 have been observed in patients with a rare dominant hair disease, monilethrix. [provided by RefSeq, Jul 2008]

Uniprot Description

K81: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13

Cellular Component: extracellular space; keratin filament

Molecular Function: protein binding; structural molecule activity

Disease: Monilethrix

Research Articles on KRT81

Similar Products

Product Notes

The KRT81 krt81 (Catalog #AAA6009592) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The KRT81 (Keratin, Type II Cuticular Hb1, Hair Keratin K2.9, Keratin, Hair, Basic, 1, Keratin-81, K81, Metastatic Lymph Node 137 Gene Protein, MLN 137, Type II Hair Keratin Hb1, Type-II Keratin Kb21, ghHKb1, ghHb1, KRTHB1, MLN137) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT81 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. Dilution: Immunofluorescence: 10ug/ml Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml. Researchers should empirically determine the suitability of the KRT81 krt81 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT81, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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