Rabbit anti-Human ADORA1 Polyclonal Antibody | anti-ADORA1 antibody

ADORA1, CT (ADORA1, Adenosine receptor A1)

Cat. Num. AAA6009437

• Gene Names: ADORA1; RDC7
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: ADORA1; Polyclonal Antibody; CT (ADORA1; Adenosine receptor A1); Anti -ADORA1; anti-ADORA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-ADORA1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: ADORA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the C-terminal region of human ADORA1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADORA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ADORA1 antibody detected the ADORA1 protein (arrow).)

Related Product Information for anti-ADORA1 antibody

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene.

Product Categories/Family for anti-ADORA1 antibody

Antibodies; Abs to Receptors

NCBI and Uniprot Product Information

• NCBI GI #: 4501947
• NCBI GeneID: 134
• NCBI Accession #: NP_000665.1
• NCBI GenBank Nucleotide #: NM_000674.2
• UniProt Accession #: P30542; Q6FHK3; Q8TAM8; A6NFY5; A6NGP4; A8K1L3; B3KXQ4
• Molecular Weight: 36,512 Da
• NCBI Official Full Name: adenosine receptor A1
• NCBI Official Synonym Full Names: adenosine A1 receptor
• NCBI Official Symbol: ADORA1
• NCBI Official Synonym Symbols: RDC7
• NCBI Protein Information: adenosine receptor A1
• UniProt Protein Name: Adenosine receptor A1
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA1
• UniProt Entry Name: AA1R_HUMAN

NCBI Description

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Receptor, GPCR; Membrane protein, integral; GPCR, family 1; Membrane protein, multi-pass; Apoptosis

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: endoplasmic reticulum; integral to plasma membrane; basolateral plasma membrane; postsynaptic density; dendritic spine; terminal button; axolemma; asymmetric synapse; presynaptic membrane; postsynaptic membrane; cell soma; plasma membrane; presynaptic active zone

Molecular Function: protein binding; G-protein-coupled receptor binding; protein heterodimerization activity; G-protein beta/gamma-subunit binding; adenosine receptor activity, G-protein coupled; purine nucleoside binding; phospholipase C activity; heterotrimeric G-protein binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; relaxation of vascular smooth muscle; negative regulation of glutamate secretion; activation of MAPKK activity; negative regulation of hormone secretion; positive regulation of potassium ion transport; regulation of synaptic plasticity; positive regulation of peptide secretion; neurological control of breathing; thermoregulation; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of glomerular filtration; positive regulation of epidermal growth factor receptor activity; cell-cell signaling; negative regulation of lipid catabolic process; positive regulation of nucleoside transport; negative regulation of vasodilation; negative regulation of blood pressure; inflammatory response; detection of temperature stimulus involved in sensory perception of pain; protein targeting to membrane; negative regulation of synaptic transmission, GABAergic; adenosine receptor signaling pathway; nervous system development; negative regulation of neurotrophin production; negative regulation of acute inflammatory response; negative regulation of synaptic transmission, glutamatergic; negative regulation of leukocyte migration; phagocytosis; response to hypoxia; regulation of sensory perception of pain; G-protein signaling, adenylate cyclase inhibiting pathway; cognition; regulation of excitatory postsynaptic membrane potential; positive regulation of blood pressure; lipid catabolic process; negative regulation of apoptosis