Rabbit anti-Human ADORA1 Polyclonal Antibody | anti-ADORA1 antibody

ADORA1, CT (ADORA1, Adenosine receptor A1) (FITC)

Cat. Num. AAA6271852

• Gene Names: ADORA1; RDC7
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ADORA1; Polyclonal Antibody; CT (ADORA1; Adenosine receptor A1) (FITC); Adenosine receptor A1; anti-ADORA1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-ADORA1 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: ADORA1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 297-326 amino acids from the C-terminal region of human ADORA1.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ADORA1 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the ADORA1 antibody detected the ADORA1 protein (arrow).)

Related Product Information for anti-ADORA1 antibody

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene.

Product Categories/Family for anti-ADORA1 antibody

Antibodies; Receptors

References

Lane, J.R., et al. Biochem. Pharmacol. 80(8):1180-1189(2010). Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010). Wagner, A.K., et al. Epilepsy Res. 90(3):259-272(2010). Ruano, G., et al. Pharmacogenomics 11(7):959-971(2010). Pinheiro, A.P., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 115305569
• NCBI GeneID: 134
• NCBI Accession #: NP_001041695.1; NP_000665.1
• NCBI GenBank Nucleotide #: NM_001048230.1
• UniProt Accession #: P30542; Q6FHK3; Q8TAM8; A6NFY5; A6NGP4; A8K1L3; B3KXQ4; D2CGD0
• Molecular Weight: 13,348 Da
• NCBI Official Full Name: adenosine receptor A1
• NCBI Official Synonym Full Names: adenosine A1 receptor
• NCBI Official Symbol: ADORA1
• NCBI Official Synonym Symbols: RDC7
• NCBI Protein Information: adenosine receptor A1
• UniProt Protein Name: Adenosine receptor A1
• Protein Family: Adenosine receptor
• UniProt Gene Name: ADORA1
• UniProt Entry Name: AA1R_HUMAN

NCBI Description

The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

ADORA1: Receptor for adenosine. The activity of this receptor is mediated by G proteins which inhibit adenylyl cyclase. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Apoptosis; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: asymmetric synapse; presynaptic membrane; postsynaptic membrane; cell soma; endoplasmic reticulum; basolateral plasma membrane; integral to plasma membrane; postsynaptic density; presynaptic active zone; plasma membrane; dendritic spine; terminal button; axolemma

Molecular Function: protein binding; G-protein-coupled receptor binding; protein heterodimerization activity; G-protein beta/gamma-subunit binding; purine nucleoside binding; adenosine receptor activity, G-protein coupled; phospholipase C activity; heterotrimeric G-protein binding

Biological Process: negative regulation of circadian sleep/wake cycle, non-REM sleep; relaxation of vascular smooth muscle; activation of MAPKK activity; negative regulation of glutamate secretion; negative regulation of hormone secretion; positive regulation of potassium ion transport; regulation of synaptic plasticity; positive regulation of peptide secretion; neurological control of breathing; thermoregulation; signal transduction; positive regulation of protein amino acid dephosphorylation; negative regulation of cell proliferation; regulation of glomerular filtration; cell-cell signaling; positive regulation of nucleoside transport; positive regulation of epidermal growth factor receptor activity; negative regulation of lipid catabolic process; negative regulation of vasodilation; negative regulation of blood pressure; detection of temperature stimulus involved in sensory perception of pain; protein targeting to membrane; inflammatory response; negative regulation of synaptic transmission, GABAergic; adenosine receptor signaling pathway; nervous system development; negative regulation of neurotrophin production; negative regulation of synaptic transmission, glutamatergic; negative regulation of acute inflammatory response; negative regulation of leukocyte migration; phagocytosis; response to hypoxia; G-protein signaling, adenylate cyclase inhibiting pathway; regulation of sensory perception of pain; regulation of excitatory postsynaptic membrane potential; cognition; positive regulation of blood pressure; lipid catabolic process; negative regulation of apoptosis