Western Blot (WB)
(Western blot analysis of anti-CNK Pab in SK-BR3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B). CNK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Rabbit anti-Human, Mouse PLK3 Polyclonal Antibody | anti-PLK3 antibody
PLK3, CT (PLK3, CNK, FNK, PRK, Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, FGF-inducible kinase, Polo-like kinase 3, Proliferation-related kinase)
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
PLK3; Polyclonal Antibody; CT (PLK3; CNK; FNK; PRK; Serine/threonine-protein kinase PLK3; Cytokine-inducible serine/threonine-protein kinase; FGF-inducible kinase; Polo-like kinase 3; Proliferation-related kinase); Anti -PLK3; anti-PLK3 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-PLK3 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow cytometry
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow cytometry
Immunogen
PLK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 617~646 amino acids from the C-terminal region of human PLK3.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of anti-CNK Pab in SK-BR3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B). CNK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Western Blot (WB)
(Western blot analysis of anti-CNK Pab in SK-BR3 cell lysate (Lane A) and mouse heart tissue lysate (Lane B). CNK (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Related Product Information for anti-PLK3 antibody
CNK, a member of the CDC5/Polo subfamily of Ser/Thr protein kinases, is involved in regulating M phase functions during the cell cycle. It may also be part of the signaling network controlling cellular adhesion. In vitro, is able to phosphorylate CDC25C and casein. This membrane-associated protein binds to the calcium/integrin-binding protein (CIB). This interaction probably occurs via the POLO-box domain. Transcripts are highly detected in placenta, lung, followed by skeletal muscle, heart, pancreas, ovaries and kidney and weakly detected in liver and brain. This protein exhibits a short half-live. In cells of hematopoietic origin, CNK is strongly and exclusively detected in terminally differentiated macrophages. Transcript expression appears to be down-regulated in primary lung tumor. Cytokine and cellular adhesion trigger CNK induction. CNK is thought to be phosphorylated as cells enter mitosis and dephosphorylated as cells exit mitosis The protein contains 2 POLO box domains.
Product Categories/Family for anti-PLK3 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
serine/threonine-protein kinase PLK3
NCBI Official Symbol
PLK3
NCBI Protein Information
polo-like kinase 3
Protein Family
Similar Products
Product Notes
The PLK3 (Catalog #AAA6009386) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PLK3, CT (PLK3, CNK, FNK, PRK, Serine/threonine-protein kinase PLK3, Cytokine-inducible serine/threonine-protein kinase, FGF-inducible kinase, Polo-like kinase 3, Proliferation-related kinase) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PLK3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow cytometry. Researchers should empirically determine the suitability of the PLK3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLK3, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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