Goat RANBP8 Polyclonal Antibody

RANBP8 (IPO8, Importin-8, Imp8, RAN-binding Protein 8, RanBP8)

Cat. Num. AAA6009158

• Reactivity: Bovine, Canine, Human, Porcine
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: RANBP8; Polyclonal Antibody; RANBP8 (IPO8; Importin-8; Imp8; RAN-binding Protein 8; RanBP8); Anti -RANBP8 (IPO8; anti-RANBP8 antibody

• Host: Goat
• Reactivity: Bovine, Canine, Human, Porcine
• Clonality: Polyclonal
• Specificity: Recognizes human IPO8. Species sequence homology: Bovine, canine and porcine.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.

• Applicable Applications for anti-RANBP8 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:32,000; Immunohistochemistry (Paraffin): 4-6ug/ml
• Immunogen: Synthetic peptide corresponding to C-SAFNFGTVPSNN, from IPO8, at C-terminal (NP_006381.2, NP_001177924.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

((4ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining. Similar results were obtained after antigen retrieval at pH9.)

Related Product Information for anti-RANBP8 antibody

Importin 8 is a 1037aa nuclear protein with a RAN binding site. It belongs to RAN-binding protein super family and hence shares an N-terminal sequence motif with importin-beta that appears to account for RAN GTP binding. It binds to the nuclear pore complex (NPC) and is well known as a transport carrier that mediates nuclear import of ribosomal proteins with a classical nuclear localization signal. Importin 8 works by binding to the nuclear pore complex and, along with RANGTP and RANBP1, inhibits the GAP stimulation of the RAN GTPase. Along with transportin it also helps in the nuclear import of SRP19.

Product Categories/Family for anti-RANBP8 antibody

Antibodies; Abs to Nuclear Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 2337918
• NCBI Official Full Name: RANBP8

NCBI Description

The importin-alpha/beta complex and the GTPase Ran mediate nuclear import of proteins with a classical nuclear localization signal. The protein encoded by this gene is a member of a class of approximately 20 potential Ran targets that share a sequence motif related to the Ran-binding site of importin-beta. This protein binds to the nuclear pore complex and, along with RanGTP and RANBP1, inhibits the GAP stimulation of the Ran GTPase. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

IPO8: Seems to function in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter- like protein in association with the importin-beta subunit KPNB1. Acting autonomously, is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro mediates the nuclear import of SRP19. Belongs to the importin beta family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear import; Nuclear export; Karyopherin; G protein regulator, misc.

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: nucleoplasm; cytosol

Molecular Function: protein binding; Ran GTPase binding

Biological Process: intracellular protein transport; gene expression; signal transduction