Mouse anti-Human CXCR7 Monoclonal Antibody | anti-CXCR7 antibody

CXCR7 (C-X-C Chemokine Receptor 7, Chemokine Orphan Receptor 1, RDC-1)

Cat. Num. AAA600631

• Gene Names: CXCR7; RDC1; CMKOR1; GPR159
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: CXCR7; Monoclonal Antibody; CXCR7 (C-X-C Chemokine Receptor 7; Chemokine Orphan Receptor 1; RDC-1); Anti -CXCR7 (C-X-C Chemokine Receptor 7; anti-CXCR7 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 8B9
• Specificity: Recognizes human CXCR7. Reacts with five distinct human CXCR7 transfectants, but not their respective parental lines. Also reacts with MCF-7 cells expression of CXCR7.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from PBS, 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

• Applicable Applications for anti-CXCR7 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Dilution: Flow Cytometry: 2.5ug labels 10e6 cells in the MCF-7 human breast cancer cell line.; Immunohistochemistry (Paraffin): 8-25ug/ml. Detected CXCR7 in paraffin sections of nude mice injected with human breast cancer cells.
• Immunogen: Human CXCR7 encoding plasmid (Accession #P25106)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Flow Cytometry (FC/FACS)

(Detection of CXCR7/RDC‑1 in MCF‑7 Human Cell Line by Flow Cytometry. MCF‑7 human breast cancer cell line was stained with MBS600631, (filled histogram) or isotype control antibody (open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')2 Secondary Antibody.)

Immunohistochemistry (IHC)

(CXCR7/RDC‑1 in Human Breast Cancer Tissue in a Mouse Model. CXCR7/RDC‑1 was detected in perfusion fixed paraffin-embedded sections of human breast cancer tissue in a mouse model using MBS600631 at 25ug/mL overnight at 4°C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).)

Related Product Information for anti-CXCR7 antibody

The G protein-coupled receptor, RDC1, belongs to a subgroup of chemokine receptors and has been designated CXCR7. CXCR7 can bind with high-affinity to CXCL12/SDF-1 and CXCL11/I-TAC. It is also a co-receptor for several HIV and SIV strains. In their N-termini and extracellular loops 1, 2 and 3, human and mouse CXCR7 share 84%, 100%, 96% and 86% amino acid sequence identity, respectively. Reports of mRNA levels and/or protein expression indicate that CXCR7 occurs on a wide variety of tissues and cells including monocytes, B cells, T cells and mature dendritic cells. In contrast, based on ligand binding analysis and receptor level (assessed using anti-CXCR7, clone 11G8), surface expression of CXCR7 was reported to be restricted to tumor cells, activated endothelial cells, fetal liver cells and few other cell types. The basis of these inconsistent observations is not known but may be attributed to cell context and the use of different antibodies that may recognize different epitopes.

Product Categories/Family for anti-CXCR7 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 31083344
• NCBI GeneID: 57007
• NCBI Accession #: NP_064707.1
• NCBI GenBank Nucleotide #: NM_020311.2
• UniProt Accession #: P25106; Q53RV4; Q8NE10; Q92938; Q92986; A8K6J4
• Molecular Weight: 41,493 Da
• NCBI Official Full Name: C-X-C chemokine receptor type 7
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) receptor 7
• NCBI Official Symbol: CXCR7
• NCBI Official Synonym Symbols: RDC1; CMKOR1; GPR159
• NCBI Protein Information: C-X-C chemokine receptor type 7; RDC-1; CXC-R7; CXCR-7; OTTHUMP00000164411; G protein-coupled receptor; chemokine orphan receptor 1; G-protein coupled receptor 159; G-protein coupled receptor RDC1 homolog
• UniProt Protein Name: C-X-C chemokine receptor type 7
• UniProt Gene Name: CXCR7
• UniProt Synonym Gene Names: CMKOR1; GPR159; RDC1
• UniProt Entry Name: CXCR7_HUMAN

NCBI Description

This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas. [provided by RefSeq]

Uniprot Description

CMKOR1: Receptor for chemokines CXCL12/SDF1 and CXCL11. Does not elicit classical chemokine receptor signaling; chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway. Acts as a scavenger for CXCL12/SDF1 and, to a lesser extent, for CXCL11. Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness. In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival. Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration. Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12. Required for heart valve development. Acts as coreceptor with CXCR4 for a restricted number of HIV isolates. Belongs to the G-protein coupled receptor 1 family.

Protein type: Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1; Membrane protein, integral

Chromosomal Location of Human Ortholog: 2q37.3

Cellular Component: recycling endosome; cell surface; perinuclear region of cytoplasm; early endosome; plasma membrane; integral to membrane; coated pit; endosome

Molecular Function: C-X-C chemokine binding; protein binding; coreceptor activity; scavenger receptor activity; C-X-C chemokine receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; viral reproduction; receptor internalization; angiogenesis; chemotaxis; vasculogenesis; cell adhesion