Goat anti-Human EYA4 Polyclonal Antibody | anti-EYA4 antibody

EYA4, NT (Eyes Absent Homolog 4)

Cat. Num. AAA6006001

• Gene Names: EYA4; CMD1J; DFNA10
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: EYA4; Polyclonal Antibody; NT (Eyes Absent Homolog 4); Anti -EYA4; anti-EYA4 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human EYA4. Species sequence homology: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-EYA4 antibody: ELISA (EL/EIA), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in ELISA and Immunohistochemistry.; Dilution: ELISA: 1:128,000; Immunohistochemistry (Formalin fixed paraffin embedded): 3.75ug/ml
• Immunogen: Synthetic peptide EDSQDLNEQSVKKTC from the N-terminus of human EYA4.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (IHC)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-EYA4 antibody

EYA4 is thought to play a role in transcription regulation during organogenesis through its intrinsic protein phosphatase activity. It may be involved in development of the eye and for continued function of the mature organ of Corti. The haloacid dehalogenase domain at the C terminus of Eya4 contains the tyrosine-phosphatase activity (that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph)), and the N-terminal half carries the threonine-phosphatase activity. 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. EYA4 promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. Mutations of the threonine-phosphatase, but not the tyrosine-phosphatase, abolished the ability of Eya4 to enhance the innate immune response, suggesting that EYA proteins regulate the innate immune response by modulating the phosphorylation state of signal transducers for intracellular pathogens. Mutations in EYA4 are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant nonsyndromic sensorineural 10 locus. Defects in this gene are also associated with dilated cardiomyopathy 1J. Four transcript variants encoding distinct isoforms have been identified.

Product Categories/Family for anti-EYA4 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 98991763
• NCBI GeneID: 2070
• NCBI Accession #: NP_742101.2
• NCBI GenBank Nucleotide #: NM_172103.3
• UniProt Accession #: O95677; O95464; O95679; Q8IW39; Q9NTR7; B7Z7F7
• Molecular Weight: 69,505 Da
• NCBI Official Full Name: eyes absent homolog 4 isoform b
• NCBI Official Synonym Full Names: eyes absent homolog 4 (Drosophila)
• NCBI Official Symbol: EYA4
• NCBI Official Synonym Symbols: CMD1J; DFNA10
• NCBI Protein Information: eyes absent homolog 4; dJ78N10.1 (eyes absent)
• UniProt Protein Name: Eyes absent homolog 4
• Protein Family: Eyes absent
• UniProt Gene Name: EYA4
• UniProt Entry Name: EYA4_HUMAN

NCBI Description

This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may act as a transcriptional activator through its protein phosphatase activity, and it may be important for eye development, and for continued function of the mature organ of Corti. Mutations in this gene are associated with postlingual, progressive, autosomal dominant hearing loss at the deafness, autosomal dominant nonsyndromic sensorineural 10 locus. Defects in this gene are also associated with dilated cardiomyopathy 1J. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

EYA4: Tyrosine phosphatase that specifically dephosphorylates 'Tyr-142' of histone H2AX (H2AXY142ph). 'Tyr-142' phosphorylation of histone H2AX plays a central role in DNA repair and acts as a mark that distinguishes between apoptotic and repair responses to genotoxic stress. Promotes efficient DNA repair by dephosphorylating H2AX, promoting the recruitment of DNA repair complexes containing MDC1. Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis. May be involved in development of the eye. Defects in EYA4 are the cause of deafness autosomal dominant type 10 (DFNA10). DFNA10 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Defects in EYA4 are the cause of cardiomyopathy dilated type 1J (CMD1J). Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death. Belongs to the HAD-like hydrolase superfamily. EYA family. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.1.3.48; Protein phosphatase, tyrosine (non-receptor)

Chromosomal Location of Human Ortholog: 6q23

Cellular Component: cytoplasm; nucleus

Molecular Function: protein binding; metal ion binding; protein tyrosine phosphatase activity

Biological Process: anatomical structure morphogenesis; sensory perception of sound; regulation of transcription, DNA-dependent; transcription, DNA-dependent; visual perception; multicellular organismal development; chromatin modification; DNA repair

Disease: Cardiomyopathy, Dilated, 1j; Deafness, Autosomal Dominant 10