Rabbit anti-Human, Mouse APITD1 Polyclonal Antibody | anti-Apitd1 antibody

APITD1, ID (APITD1, CENPS, FAAP16, MHF1, Centromere protein S, Apoptosis-inducing TAF9-like domain-containing protein 1, FANCM-interacting histone fold protein 1, Fanconi anemia-associated polypeptide of 16kD)

Cat. Num. AAA6005280

• Gene Names: Apitd1; CENP-S; 2610040C18Rik; 2810407L01Rik
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: APITD1; Polyclonal Antibody; ID (APITD1; CENPS; FAAP16; MHF1; Centromere protein S; Apoptosis-inducing TAF9-like domain-containing protein 1; FANCM-interacting histone fold protein 1; Fanconi anemia-associated polypeptide of 16kD); Anti -APITD1; anti-Apitd1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Apitd1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: APITD1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between amino acid 50-80 from the Central region of human APITD1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane) using MBS6005280. APITD1 (arrow) was detected using MBS6005280.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded brain tissue using MBS6005280 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6005280 for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells (right histogram) using MBS6005280 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-Apitd1 antibody

APITD1 was identified in the neuroblastoma tumour suppressor candidate region on chromosome 1p36. It contains a TFIID-31 domain, similar to that found in TATA box-binding protein-associated factor, TAF(II)31, which is required for p53-mediated transcription activation. This gene was expressed at very low levels in neuroblastoma tumours, and was shown to reduce cell growth in neuroblastoma cells, suggesting that it may have a role in a cell death pathway.

Product Categories/Family for anti-Apitd1 antibody

Antibodies; Abs to Fanconi Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 28913612
• NCBI GeneID: 69928
• UniProt Accession #: Q9D084; Q80ZS2; Q8BH28; A2AH77
• Molecular Weight: 16,335 Da
• NCBI Official Full Name: Apitd1 protein
• NCBI Official Synonym Full Names: apoptosis-inducing, TAF9-like domain 1
• NCBI Official Symbol: Apitd1
• NCBI Official Synonym Symbols: CENP-S; 2610040C18Rik; 2810407L01Rik
• NCBI Protein Information: centromere protein S; FANCM-interacting histone fold protein 1; ortholog of human apoptosis-inducing, TAF9-like domain 1 APITD1; apoptosis-inducing TAF9-like domain-containing protein 1 homolog
• UniProt Protein Name: Centromere protein S
• Protein Family: Centromere protein
• UniProt Gene Name: Apitd1
• UniProt Synonym Gene Names: Cenps; FAAP16; MHF1; CENP-S
• UniProt Entry Name: CENPS_MOUSE

Uniprot Description

APITD1: DNA-binding component of the FA core complex involved in DNA damage repair and genome maintenance. Required for optimal chromatin association of the FA core complex. Required for efficient damage-induced monoubiquitination and focus formation of FANCD2. Stabilizes FAAD24, FANCM and STRA13/CENPX in the FA core complex. Plays a role in DNA interstrand cross-linking (ICL) repair and in recovery of replication forks stalled by topoisomerase I-DNA cleavage intermediates induced by camptothecin. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. Component of the APITD1/CENPS complex that is essential for the stable assembly of the outer kinetochore. Plays an important role in mitotic progression and chromosome segregation. Component of the CENPA-CAD (nucleosome distal) complex, a complex recruited to centromeres which is involved in assembly of kinetochore proteins, mitotic progression and chromosome segregation. Belongs to the TAF9 family. 3 isoforms of the human protein are produced by alternative splicing.

Cellular Component: kinetochore; chromosome; nucleus; chromosome, pericentric region

Molecular Function: DNA binding; protein heterodimerization activity; double-stranded DNA binding; chromatin binding

Biological Process: mitosis; cell division; cell cycle; DNA repair; response to DNA damage stimulus