Rabbit anti-Human, Mouse Mapk11 Polyclonal Antibody | anti-MAPK11 antibody

Mapk11, NT (Mapk11, Prkm11, Mitogen-activated protein kinase 11, Mitogen-activated protein kinase p38 beta)

Cat. Num. AAA6005278

• Gene Names: MAPK11; P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: Mapk11; Polyclonal Antibody; NT (Mapk11; Prkm11; Mitogen-activated protein kinase 11; Mitogen-activated protein kinase p38 beta); Anti -Mapk11; anti-MAPK11 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-MAPK11 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50
• Immunogen: Mouse Mapk11 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human Mouse Mapk11.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Mapk11 Antibody (N-term) western blot analysis in Mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Mapk11 antibody detected the Mapk11 protein (arrow).)

Western Blot (WB)

(Mouse Mapk11 Antibody (N-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the Mapk11 antibody detected the Mapk11 protein (arrow).)

Immunohistochemistry (IHC)

(Mouse Mapk11 Antibody (N-term) (AP13920a)immunohistochemistry analysis in formalin fixed and paraffin embedded mouse live tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Mouse Mapk11 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-MAPK11 antibody

Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2 (By similarity).

Product Categories/Family for anti-MAPK11 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 47678559
• NCBI GeneID: 5600
• UniProt Accession #: Q15759; O00284; O15472; Q2XNF2; B0LPG1
• Molecular Weight: 41,357 Da
• NCBI Official Full Name: MAPK11
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 11
• NCBI Official Symbol: MAPK11
• NCBI Official Synonym Symbols: P38B; SAPK2; p38-2; PRKM11; SAPK2B; p38Beta; P38BETA2
• NCBI Protein Information: mitogen-activated protein kinase 11; MAPK 11; MAP kinase 11; MAP kinase p38 beta; stress-activated protein kinase 2; stress-activated protein kinase-2; stress-activated protein kinase 2b; stress-activated protein kinase-2b; mitogen-activated protein kinase p38-2; mitogen-activated protein kinase p38 beta
• UniProt Protein Name: Mitogen-activated protein kinase 11
• UniProt Gene Name: MAPK11
• UniProt Synonym Gene Names: PRKM11; SAPK2; SAPK2B; MAP kinase 11; MAPK 11; MAP kinase p38 beta; p38b; SAPK2b
• UniProt Entry Name: MK11_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. This kinase is most closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and environmental stress. This kinase is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase. [provided by RefSeq, Jul 2008]

Uniprot Description

P38B: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Inhibited by SB203580 and pyridinyl-imidazole related compounds.

Protein type: EC 2.7.11.24; Kinase, protein; Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/p38 subfamily; p38 subfamily

Chromosomal Location of Human Ortholog: 22q13.33

Cellular Component: nucleoplasm; cytosol

Molecular Function: MAP kinase activity; protein serine/threonine kinase activity; protein binding; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of transcription factor activity; response to stress; toll-like receptor 4 signaling pathway; mitochondrion organization and biogenesis; transcription, DNA-dependent; MyD88-independent toll-like receptor signaling pathway; organelle organization and biogenesis; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; muscle cell differentiation; Ras protein signal transduction; toll-like receptor signaling pathway; innate immune response; positive regulation of muscle cell differentiation; gene expression; toll-like receptor 9 signaling pathway; vascular endothelial growth factor receptor signaling pathway