Mouse anti-Human, Mouse Lymphoid Enhancer Factor 1 Monoclonal Antibody | anti-LEF1 antibody

Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)

Cat. Num. AAA600490

• Gene Names: LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA
• Reactivity: Human, Mouse
• Applications: Western Blot, Immunocytochemistry
• Purity: Highly Purified; Purified by size exclusion chromatography.
• Synonyms: Lymphoid Enhancer Factor 1; Monoclonal Antibody; Lymphoid Enhancer Factor 1 (LEF1; Lymphoid Enhancer-binding Factor 1; DKFZp586H0919; HGNC:6551; T cell Specific Transcription Factor 1 alpha; TCF1 alpha); Anti -Lymphoid Enhancer Factor 1 (LEF1; anti-LEF1 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6D538
• Specificity: Recognizes human and mouse LEF. Does not cross-react with T cell factor 3 (TCF3) or T cell factor 4 (TCF4). Detects a band of ~68kDa by Western blot.
• Purity/Purification: Highly Purified; Purified by size exclusion chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.1% sodium azide, PEG and sucrose, before the addiition of glycerol to 40%.

• Applicable Applications for anti-LEF1 antibody: Western Blot (WB), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Immunocytochemistry and Western Blot.; Dilution: Western Blot: 1ug/ml for AP/BCIP?NBT (MTT) detection; 0.1ug/ml for HRPO/ECL detection.; Immunocytochemistry: 1ug/ml in PBS
• Immunogen: Recombinant LEF (Lymphoid Enhancer Binding Factor) (aa 1-85).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for anti-LEF1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7705917
• NCBI GeneID: 51176
• NCBI Accession #: NP_057353.1
• NCBI GenBank Nucleotide #: NM_016269.4
• UniProt Accession #: Q9UJU2; Q3ZCU4; Q9HAZ0; B4DG38; B7Z8E2; E9PDK3
• Molecular Weight: 44,201 Da
• NCBI Official Full Name: lymphoid enhancer-binding factor 1 isoform 1
• NCBI Official Synonym Full Names: lymphoid enhancer-binding factor 1
• NCBI Official Symbol: LEF1
• NCBI Official Synonym Symbols: LEF-1; TCF10; TCF7L3; TCF1ALPHA
• NCBI Protein Information: lymphoid enhancer-binding factor 1; TCF1-alpha; T cell-specific transcription factor 1-alpha
• UniProt Protein Name: Lymphoid enhancer-binding factor 1
• Protein Family: Lymphoid enhancer-binding factor
• UniProt Gene Name: LEF1
• UniProt Synonym Gene Names: LEF-1; TCF1-alpha
• UniProt Entry Name: LEF1_HUMAN

NCBI Description

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

LEF-1: a transcription factor of the TCF/LEF family. Participates in the Wnt signaling pathway and regulates T-cell receptor alpha-C enhancer function. Activates transcription of target genes in the presence of CTNNB1 and EP300. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. Deletions in LEF1 have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Four human isoforms produced by alternative promoter usage and alternative splicing have been described. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 4q23-q25

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; estrogen receptor activity; histone binding; caspase inhibitor activity; beta-catenin binding; transcription factor binding; protein binding; DNA binding; sequence-specific DNA binding; gamma-catenin binding; estrogen receptor binding; chromatin binding; transcription factor activity; DNA bending activity

Biological Process: radial glial cell differentiation in the forebrain; hypothalamus development; positive regulation of transcription, DNA-dependent; T-helper 1 cell differentiation; Wnt receptor signaling pathway through beta-catenin; forebrain neuroblast division; forebrain neuron differentiation; BMP signaling pathway; negative regulation of interleukin-13 production; neural crest cell migration; regulation of striated muscle development; embryonic limb morphogenesis; somitogenesis; dentate gyrus development; sensory perception of taste; negative regulation of striated muscle development; positive regulation of cell growth; patterning of blood vessels; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis; B cell proliferation; transcription from RNA polymerase II promoter; tongue development; negative regulation of interleukin-4 production; paraxial mesoderm formation; positive regulation of granulocyte differentiation; alpha-beta T cell differentiation; palate development; negative regulation of transcription from RNA polymerase II promoter; negative regulation of caspase activity; anatomical structure regression; regulation of cell-cell adhesion; mammary gland development; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; muscle fiber development; Wnt receptor signaling pathway; neutrophil differentiation; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; formation of radial glial scaffolds; osteoblast differentiation; T cell receptor V(D)J recombination; epithelial to mesenchymal transition; sprouting angiogenesis; negative regulation of cell-cell adhesion; eye pigmentation; positive regulation of cell migration