Rabbit Lymphoid Enhancer Factor 1 Monoclonal Antibody | anti-LEF1 antibody

Lymphoid Enhancer Factor 1 (LEF1, Lymphoid Enhancer-binding Factor 1, DKFZp586H0919, HGNC:6551, T cell Specific Transcription Factor 1 alpha, TCF1 alpha)

Cat. Num. AAA608415

• Gene Names: LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Supernatant; Supernatant
• Synonyms: Lymphoid Enhancer Factor 1; Monoclonal Antibody; Lymphoid Enhancer Factor 1 (LEF1; Lymphoid Enhancer-binding Factor 1; DKFZp586H0919; HGNC:6551; T cell Specific Transcription Factor 1 alpha; TCF1 alpha); Anti -Lymphoid Enhancer Factor 1 (LEF1; anti-LEF1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG
• Clone Number: 7H38
• Specificity: Recognizes endogenous level of total human LEF1 protein at ~25-58kD. Does not recognize the dominant negative forms of LEF1 generated by an alternative promoter.
• Purity/Purification: Supernatant; Supernatant
• Form/Format: Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, < 0.02% sodium azide, 50% glycerol.

• Applicable Applications for anti-LEF1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Flow Cytometry, Immunofluorescence and Immunocytochemistry.; Dilution: Western Blot: 1:1000. Incubate membrane with diluted antibody in 1X TBS, 5% BSA, 0.1% Tween-20 at 4ºC with gentle shaking, overnight.; Immunoprecipitation: 1:50; Flow Cytometry: 1:200; Immunofluorescence (IF-IC): 1:100
• Immunogen: Synthetic peptide corresponding to residues surrounding Pro82 of human LEF1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of total cell lysates from HCT15, DLD1 and mouse thymocytes using MBS608415.)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of untreated Jurkat cells using MBS608415 (blue) compared to a nonspecific negative control antibody (red).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of HCT-15 cells using MBS608415 (green) and Pan-Keratin Mouse mAb (red).)

Related Product Information for anti-LEF1 antibody

LEF1/TCF are members of the high mobility group (HMG) DNA binding protein family of transcription factors which consists of four members: Lymphoid enhancer factor 1 (LEF1), T Cell Factor 1 (TCF1), TCF3 and TCF4 (1). LEF1 and TCF1 were originally identified as important factors regulating early lymphoid development (2) and are downstream factors in the Wnt signaling pathway. LEF1/TCF bind to Wnt response elements to provide a docking site for beta-catenin which translocates to the nucleus and promotes the transcription of target genes upon activation of Wnt signaling (3). LEF1/TCF proteins are dynamically expressed during development and aberrant activation of the Wnt signaling pathway is involved in many types of cancers such as colon cancers (4,5).

Product Categories/Family for anti-LEF1 antibody

Antibodies; Abs to Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 7705917
• NCBI GeneID: 51176
• NCBI Accession #: NP_057353.1
• NCBI GenBank Nucleotide #: NM_016269.4
• UniProt Accession #: Q9UJU2; Q3ZCU4; Q9HAZ0; B4DG38; B7Z8E2; E9PDK3
• Molecular Weight: 44,201 Da
• NCBI Official Full Name: lymphoid enhancer-binding factor 1 isoform 1
• NCBI Official Synonym Full Names: lymphoid enhancer-binding factor 1
• NCBI Official Symbol: LEF1
• NCBI Official Synonym Symbols: LEF-1; TCF10; TCF7L3; TCF1ALPHA
• NCBI Protein Information: lymphoid enhancer-binding factor 1; TCF1-alpha; T cell-specific transcription factor 1-alpha
• UniProt Protein Name: Lymphoid enhancer-binding factor 1
• Protein Family: Lymphoid enhancer-binding factor
• UniProt Gene Name: LEF1
• UniProt Synonym Gene Names: LEF-1; TCF1-alpha
• UniProt Entry Name: LEF1_HUMAN

NCBI Description

This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]

Uniprot Description

LEF-1: a transcription factor of the TCF/LEF family. Participates in the Wnt signaling pathway and regulates T-cell receptor alpha-C enhancer function. Activates transcription of target genes in the presence of CTNNB1 and EP300. TLE1, TLE2, TLE3 and TLE4 repress transactivation mediated by LEF1 and CTNNB1. Binds DNA in a sequence-specific manner. PIAG antagonizes both Wnt-dependent and Wnt-independent activation by LEF1. Deletions in LEF1 have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Four human isoforms produced by alternative promoter usage and alternative splicing have been described. Isoform 3 lacks the CTNNB1 interaction domain and may be an antagonist for Wnt signaling.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 4q23-q25

Cellular Component: nucleoplasm; transcription factor complex; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; estrogen receptor activity; histone binding; caspase inhibitor activity; beta-catenin binding; transcription factor binding; protein binding; DNA binding; sequence-specific DNA binding; gamma-catenin binding; estrogen receptor binding; chromatin binding; transcription factor activity; DNA bending activity

Biological Process: transcription from RNA polymerase II promoter; B cell proliferation; negative regulation of interleukin-4 production; tongue development; radial glial cell differentiation in the forebrain; hypothalamus development; paraxial mesoderm formation; positive regulation of transcription, DNA-dependent; positive regulation of granulocyte differentiation; T-helper 1 cell differentiation; alpha-beta T cell differentiation; negative regulation of transcription from RNA polymerase II promoter; Wnt receptor signaling pathway through beta-catenin; negative regulation of caspase activity; palate development; forebrain neuroblast division; anatomical structure regression; forebrain neuron differentiation; BMP signaling pathway; negative regulation of interleukin-13 production; regulation of cell-cell adhesion; mammary gland development; positive regulation of cell proliferation; positive regulation of cell-cell adhesion; muscle fiber development; neural crest cell migration; embryonic limb morphogenesis; regulation of striated muscle development; somitogenesis; Wnt receptor signaling pathway; dentate gyrus development; sensory perception of taste; neutrophil differentiation; negative regulation of striated muscle development; positive regulation of cell growth; negative regulation of DNA binding; odontogenesis of dentine-containing teeth; osteoblast differentiation; patterning of blood vessels; formation of radial glial scaffolds; T cell receptor V(D)J recombination; epithelial to mesenchymal transition; negative regulation of interleukin-5 production; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; negative regulation of cell-cell adhesion; sprouting angiogenesis; negative regulation of transcription, DNA-dependent; eye pigmentation; negative regulation of apoptosis; positive regulation of cell migration