Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS6004665.)
Rabbit anti-Human, Rat GFPT1 Monoclonal Antibody | anti-GFPT1 antibody
GFPT1 (Glucosamine-fructose-6-phosphate Aminotransferase [Isomerizing] 1, GFPT, Glutamine:fructose 6 Phosphate Amidotransferase 1, GFAT 1, GFAT1, GFAT1m, GFA, GFAT, D-fructose-6-phosphate Amidotransferase 1, Hexosephosphate Aminotransferase 1)
Purified
Purified
Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight.
Immunoprecipitation: 1:50
Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS6004665.)
Western Blot (WB)
(Western blot analysis of extracts from various cell types using MBS6004665.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the first and rate-limiting enzyme of the hexosamine pathway and controls the flux of glucose into the hexosamine pathway. The product of this gene catalyzes the formation of glucosamine 6-phosphate. [provided by RefSeq, Sep 2008]
Uniprot Description
GFAT: Controls the flux of glucose into the hexosamine pathway. Most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. Homotetramer (Potential). Isoform 1 is predominantly expressed in skeletal muscle. Not expressed in brain. Seems to be selectively expressed in striated muscle. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Carbohydrate Metabolism - amino sugar and nucleotide sugar; Amino Acid Metabolism - alanine, aspartate and glutamate; Transferase; EC 2.6.1.16
Chromosomal Location of Human Ortholog: 2p13
Cellular Component: cytosol
Molecular Function: amino acid binding; glutamine-fructose-6-phosphate transaminase (isomerizing) activity; carbohydrate binding
Biological Process: dolichol-linked oligosaccharide biosynthetic process; unfolded protein response; glucosamine biosynthetic process; glutamine metabolic process; fructose 6-phosphate metabolic process; post-translational protein modification; carbohydrate biosynthetic process; protein homotetramerization; response to sucrose stimulus; unfolded protein response, activation of signaling protein activity; cellular protein metabolic process; cellular response to insulin stimulus; energy reserve metabolic process; UDP-N-acetylglucosamine biosynthetic process; protein amino acid N-linked glycosylation via asparagine; circadian regulation of gene expression; negative regulation of glycogen biosynthetic process
Disease: Myasthenic Syndrome, Congenital, Associated With Acetylcholine Receptor Deficiency; Myasthenic Syndrome, Congenital, With Tubular Aggregates 1
Research Articles on GFPT1
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Product Notes
The GFPT1 gfpt1 (Catalog #AAA6004665) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The GFPT1 (Glucosamine-fructose-6-phosphate Aminotransferase [Isomerizing] 1, GFPT, Glutamine:fructose 6 Phosphate Amidotransferase 1, GFAT 1, GFAT1, GFAT1m, GFA, GFAT, D-fructose-6-phosphate Amidotransferase 1, Hexosephosphate Aminotransferase 1) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's GFPT1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP). Suitable for use in Western Blot and Immunoprecipitation. Dilution: Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4 degree C with gentle shaking, overnight. Immunoprecipitation: 1:50. Researchers should empirically determine the suitability of the GFPT1 gfpt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFPT1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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