Mouse anti-Human BACH1 Monoclonal Antibody | anti-BACH1 antibody

BACH1

Cat. Num. AAA600442

• Gene Names: BACH1; BACH-1; BACH1
• Reactivity: Human
• Applications: Western Blot, Immunoprecipitation, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: BACH1; Monoclonal Antibody; Anti -BACH1; anti-BACH1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 3G11
• Specificity: Recognizes the ~130kD human BACH1 protein.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.

• Applicable Applications for anti-BACH1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence.; Dilution: Western Blot: 1-3ug/ml; Immunoprecipitation: Native
• Immunogen: BACH1/GST fusion protein encompassing the C-terminus of human BACH1.
• Positive Control: MCF7, HeLa and 293 cell lysates.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Related Product Information for anti-BACH1 antibody

BRCA1 has been shown to interact with a novel member of the DEAH helicase family, BACH12. The BRCA1 gene encodes an 1863 residue nuclear protein with N-terminal RING and C-terminal BRCT domains. BACH1 appears to bind directly to these BRCT repeats of BRCA1. Moreover, BACH1 appears to contribute to the DNA repair of BRCA1. BACH1 derivative bearing a mutation in a key residue that is essential for catalytic function in other helicases interfered with normal DSBR in a manner that was dependent upon its ability to interact with BRCA12. Tumorpredisposing missense and deletion mutations in the BRCA1 BRCT domain, all of which render BRCA1 defective in its DSBR function also disrupted BACH1 binding to BRCA1. BACH1 is an integral part of BRCA1 in DSBR2. Western blots of extracts from three different cell lines revealed a band of 130kD for BACH1 in all MCF7, HeLa and HCC1937 cell line. Immunofluorescence analysis revealed the presence of punctate nuclear staining in multiple human cell lines.

Product Categories/Family for anti-BACH1 antibody

Antibodies; Abs to Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 2911116
• NCBI GeneID: 571
• UniProt Accession #: O14867; O43285; Q6ICU0; Q9BXM4
• Molecular Weight: 81,958 Da
• NCBI Official Full Name: BACH1
• NCBI Official Synonym Full Names: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• NCBI Official Symbol: BACH1
• NCBI Official Synonym Symbols: BACH-1; BACH1
• NCBI Protein Information: transcription regulator protein BACH1; HA2303; BTB and CNC homolog 1; basic region leucine zipper transcriptional regulator BACH1
• UniProt Protein Name: Transcription regulator protein BACH1
• Protein Family: Transcription regulator protein
• UniProt Gene Name: BACH1
• UniProt Entry Name: BACH1_HUMAN

NCBI Description

This gene encodes a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq]

Uniprot Description

BACH1: a transcriptional regulator that acts as a repressor or activator. Binds, in-vitro, to NF-E2 binding sites on DNA. Plays an important role in coordinating transcription activation and repression by NF-E2. Belongs to the bZIP family, CNC subfamily.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: heme binding; transcription factor activity

Biological Process: G1/S-specific transcription in mitotic cell cycle; transcription, DNA-dependent; regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; G2/M-specific transcription in mitotic cell cycle