Rabbit anti-Human, Mouse PRKAG1 Polyclonal Antibody | anti-Prkag1 antibody

PRKAG1, NT (PRKAG1, 5'-AMP-activated protein kinase subunit gamma-1)

Cat. Num. AAA6003167

• Gene Names: Prkag1; Prkaac; AA571379; BB036179
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: PRKAG1; Polyclonal Antibody; NT (PRKAG1; 5'-AMP-activated protein kinase subunit gamma-1); Anti -PRKAG1; anti-Prkag1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human, mouse
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-Prkag1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in Western Blot, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500
• Immunogen: PRKAG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 5-33 amino acids from the N-terminal region of human PRKAG1.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(PRKAG1 Antibody (N-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).)

Western Blot (WB)

(PRKAG1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the PRKAG1 antibody detected the PRKAG1 protein (arrow).)

Western Blot (WB)

(Western blot analysis of PRKAG1 (arrow) using rabbit polyclonal PRKAG1 Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRKAG1 gene.)

Related Product Information for anti-Prkag1 antibody

The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Product Categories/Family for anti-Prkag1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 83318361
• NCBI GeneID: 19082
• UniProt Accession #: O54950; Q5PRE8
• Molecular Weight: 37,520 Da
• NCBI Official Full Name: Prkag1 protein
• NCBI Official Synonym Full Names: protein kinase, AMP-activated, gamma 1 non-catalytic subunit
• NCBI Official Symbol: Prkag1
• NCBI Official Synonym Symbols: Prkaac; AA571379; BB036179
• NCBI Protein Information: 5'-AMP-activated protein kinase subunit gamma-1; AMPKg; AMPK gamma1; AMPK subunit gamma-1
• UniProt Protein Name: 5'-AMP-activated protein kinase subunit gamma-1
• Protein Family: 5'-AMP-activated protein kinase
• UniProt Gene Name: Prkag1
• UniProt Synonym Gene Names: Prkaac; AMPK gamma1; AMPK subunit gamma-1; AMPKg
• UniProt Entry Name: AAKG1_MOUSE

Uniprot Description

AMPKG1: a non-catalytic subunit of AMPK, a highly conserved kinase of the CAMKL family. The holoenzyme consists of a catalytic subunit (alpha) and two regulatory subunits (beta, gamma). Each subunit exists as multiple genetic isoforms giving rise to 12 different possible combinations of holoenzyme. Plays a key role in regulation of energy homeostasis. Activated by an elevated AMP-ATP ratio due to environmental stress, such as heat shock, hypoxia and ischemia. AMPK phosphorylates and regulates many metabolic enzymes.

Protein type: Autophagy; Protein kinase, regulatory subunit

Cellular Component: protein complex; membrane; nucleus; AMP-activated protein kinase complex

Molecular Function: AMP-activated protein kinase activity; nucleotide binding; ADP binding; protein kinase binding; ATP binding; AMP binding; protein kinase activity

Biological Process: protein heterooligomerization; regulation of catalytic activity; lipid metabolic process; fatty acid metabolic process; protein amino acid phosphorylation; fatty acid biosynthetic process