Mouse b2-Microglobulin Monoclonal Antibody | anti-HLA-G antibody

b2-Microglobulin (B2M)

Cat. Num. AAA600300

• Gene Names: HLA-G; MHC-G
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Purified
• Synonyms: b2-Microglobulin; Monoclonal Antibody; b2-Microglobulin (B2M); Anti -b2-Microglobulin (B2M); anti-HLA-G antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 1.B.749
• Specificity: Recognizes human Beta 2-microglobulin (MW 12kDa). Does not react with Beta 2-microglobulin from other species. Recognizes the HLA-Beta 2-microglobulin complex, free or associated with the membrane. The exact epitope for the antibody has not been mapped
• Purity/Purification: Purified
• Form/Format: Supplied as a lyophilized powder from PBS, 1mg/ml BSA. Reconstitute with 1ml sterile ddH2O.
• Concentration: ~0.2mg/ml (after reconstitution) (varies by lot)

• Applicable Applications for anti-HLA-G antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Immunofluorescence/Flow Cytometry: 10ul/5x10e5 cells/test.; ; Immunohistochemistry: frozen sections of cell smears): 1:25-1:50; ; Optimal dilutions to be determined by the researcher.
• Immunogen: Purified human Beta 2-microglobulin
• Hybridoma: NS1/Ag4.1 myeloma cells with spleen cells of Balb/c mice.
• Grade: Purified
• Preparation and Storage: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-HLA-G antibody

b2-microglobulin non-covalently associates with the 44kD a-chain to form the HLA Class I antigen complex. Human b2-microglobulin associated with HLA Class I antigens is expressed on many types of cells including lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells, and epithelial cells. It is absent on erythrocytes.

Product Categories/Family for anti-HLA-G antibody

Antibodies; Abs to Serum Proteins

References

Activation of complement by monoclonal antibodies that targetcell-associated 2-microglobulin: Implications for cancerimmunotherapy. Michael J. Pokrass, Monica F. Liu, Margaret A. Lindorfer, Ronald P. Taylor (2013)

NCBI and Uniprot Product Information

• NCBI GI #: 188468
• NCBI GeneID: 3135
• UniProt Accession #: O02923; O02924; O02925; O02926; O02927; O02928; O02929; O19588; O19658; O19659
• NCBI Official Full Name: b2 microglobulin
• NCBI Official Synonym Full Names: major histocompatibility complex, class I, G
• NCBI Official Symbol: HLA-G
• NCBI Official Synonym Symbols: MHC-G
• NCBI Protein Information: HLA class I histocompatibility antigen, alpha chain G; HLA G antigen; b2 microglobulin; OTTHUMP00000029218; OTTHUMP00000029220; OTTHUMP00000033527; OTTHUMP00000159507; HLA class I molecule; MHC class I antigen G; HLA-G histocompatibility antigen, class I, G
• UniProt Protein Name: HLA-G protein
• Protein Family: HLA class I histocompatibility antigen
• UniProt Gene Name: HLA-G
• UniProt Entry Name: O02923_HUMAN

NCBI Description

HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq]

Uniprot Description

Sequence similarities: Belongs to the MHC class I family. RuleBase RU004439