Mouse anti-Human, Rat RBM15 Polyclonal Antibody | anti-RBM15 antibody

RBM15 (RNA-binding Motif Protein 15, Putative RNA-binding Protein 15, One-twenty Two Protein 1, OTT1, OTT, SPEN)

Cat. Num. AAA6002439

• Gene Names: RBM15; OTT; OTT1; SPEN
• Reactivity: Human, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: RBM15; Polyclonal Antibody; RBM15 (RNA-binding Motif Protein 15; Putative RNA-binding Protein 15; One-twenty Two Protein 1; OTT1; OTT; SPEN); Anti -RBM15 (RNA-binding Motif Protein 15; anti-RBM15 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RBM15. Species Crossreactivity: rat.
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2.
• Sequence: MSHRAKLAMSGKIIIRNPIKIGYGKATPTTRLWVGGLGPWVPLAALAREFDRFGTIRTIDYRKGDSWAYIQYESLDAAHAAWTHMRGFPLGGPDRRLRVDFADTEHRYQQQYLQPLPLTHYELVTDAFGHRAPDPLRGARDRTPPLLYRDRDRDLYPDSDWVPPPPPVRERSTRTAATSVPAYEPLDSLDRRRDGWSLDRDRGDRDLPSSRDQPRKRRLPEESGGRHLDRSPESDRPRKRHCAPSPDRSPELSSSRDRYNSDNDRSSRLLLERPSPIRDRRGSLEKSQGDKRDRKNSASAERDRKHRTTAPTEGKSPLKKEDRSDGSAPSTSTASSKLKSPSQKQDGGTAPVASASPKLCLAWQGMLLLKNSNFPSNMHLLQGDLQVASSLLVEGSTGGKVAQLKITQRLRLDQPKLDEVTRRIKVAGPNGYAILLAVPGSSDSRSSSSSAASDTATSTQRPLRNLVSYLKQKQAAGVISLPVGGNKDKENTGVLHAFPPCEFSQQFLDSPAKALAKSEEDYLVMIIVRAKLVEQRMKIWNSKL

• Applicable Applications for anti-RBM15 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Suitable for use in Immunofluorescence and Western Blot.; Dilution: Immunofluorescence: 10ug/ml
• Immunogen: Full length human RBM15, aa1-544 (AAI03661.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RBM15 polyclonal antibody. Western Blot analysis of RBM15 expression in rat brain.)

Western Blot (WB)

(Western Blot analysis of RBM15 expression in transfected 293T cell line by RBM15 polyclonal antibody. Lane 1: RBM15 transfected lysate (59.84kD). Lane 2: Non-transfected lysate.)

Immunofluorescence (IF)

(Immunofluorescence of purified antibody to RBM15 on HeLa cell. [antibody concentration 10ug/ml].)

Product Categories/Family for anti-RBM15 antibody

Antibodies; Abs to RNA Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 73695337
• NCBI GeneID: 64783
• UniProt Accession #: Q96T37; Q4V760; Q5D058; Q5T613; Q96PE4; Q96SC5; Q96SC6; Q96SC9; Q96SD0; Q96T38; Q9BRA5; Q9H6R8
• Molecular Weight: 107,189 Da
• NCBI Official Full Name: RBM15 protein
• NCBI Official Synonym Full Names: RNA binding motif protein 15
• NCBI Official Symbol: RBM15
• NCBI Official Synonym Symbols: OTT; OTT1; SPEN
• NCBI Protein Information: putative RNA-binding protein 15; one twenty-two; one twenty two protein; one-twenty two protein 1; RNA-binding motif protein 15
• UniProt Protein Name: Putative RNA-binding protein 15
• Protein Family: Putative RNA-binding protein
• UniProt Gene Name: RBM15
• UniProt Synonym Gene Names: OTT; OTT1
• UniProt Entry Name: RBM15_HUMAN

NCBI Description

Members of the SPEN (Split-end) family of proteins, including RBM15, have repressor function in several signaling pathways and may bind to RNA through interaction with spliceosome components (Hiriart et al., 2005 [PubMed 16129689]).[supplied by OMIM, Feb 2009]

Uniprot Description

RBM15: May be implicated in HOX gene regulation. A chromosomal aberration involving RBM15 may be a cause of acute megakaryoblastic leukemia. Translocation t(1;22)(p13;q13) with MKL1. Although both reciprocal fusion transcripts are detected in acute megakaryoblastic leukemia (AMKL, FAB-M7), the RBM15-MKL1 chimeric protein has all the putative functional domains encoded by each gene and is the candidate oncogene. Belongs to the RRM Spen family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; Spliceosome; RNA-binding

Chromosomal Location of Human Ortholog: 1p13

Cellular Component: nucleoplasm; nuclear membrane

Molecular Function: protein binding; nucleotide binding

Biological Process: spleen development; patterning of blood vessels; negative regulation of myeloid cell differentiation; viral reproduction; negative regulation of transcription, DNA-dependent; activation of Notch receptor target transcription factor