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Western Blot (WB) (Mouse Enpp1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Mouse Enpp1 antibody detected the Mouse Enpp1 protein (arrow).)

Rabbit anti-Mouse Enpp1 Polyclonal Antibody | anti-ENPP1 antibody

Enpp1, NT (Enpp1, Npps, Pc1, Pdnp1, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, Lymphocyte antigen 41, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, Alkaline phosphodiesterase I, Nucleotide

Gene Names
ENPP1; M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; PDNP1
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
Enpp1; Polyclonal Antibody; NT (Enpp1; Npps; Pc1; Pdnp1; Ectonucleotide pyrophosphatase/phosphodiesterase family member 1; Lymphocyte antigen 41; Phosphodiesterase I/nucleotide pyrophosphatase 1; Plasma-cell membrane glycoprotein PC-1; Alkaline phosphodiesterase I; Nucleotide; Anti -Enpp1; anti-ENPP1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-ENPP1 antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in Western Blot, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunogen
Mouse Enpp1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 29-57 amino acids from the N-terminal region of mouse Enpp1.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Mouse Enpp1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Mouse Enpp1 antibody detected the Mouse Enpp1 protein (arrow).)

Western Blot (WB) (Mouse Enpp1 Antibody (N-term) western blot analysis in mouse bladder tissue lysates (35ug/lane).This demonstrates the Mouse Enpp1 antibody detected the Mouse Enpp1 protein (arrow).)
Related Product Information for anti-ENPP1 antibody
Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity (By similarity).
Product Categories/Family for anti-ENPP1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
104,924 Da
NCBI Official Full Name
ectonucleotide pyrophosphatase/phosphodiesterase family member 1
NCBI Official Synonym Full Names
ectonucleotide pyrophosphatase/phosphodiesterase 1
NCBI Official Symbol
ENPP1
NCBI Official Synonym Symbols
M6S1; NPP1; NPPS; PC-1; PCA1; ARHR2; PDNP1
NCBI Protein Information
ectonucleotide pyrophosphatase/phosphodiesterase family member 1; E-NPP 1; Ly-41 antigen; alkaline phosphodiesterase 1; plasma-cell membrane glycoprotein 1; plasma-cell membrane glycoprotein PC-1; membrane component chromosome 6 surface marker 1; phosphodiesterase I/nucleotide pyrophosphatase 1; membrane component, chromosome 6, surface marker 1
UniProt Protein Name
Ectonucleotide pyrophosphatase/phosphodiesterase family member 1
UniProt Gene Name
ENPP1
UniProt Synonym Gene Names
M6S1; NPPS; PC1; PDNP1; E-NPP 1; NPPase
UniProt Entry Name
ENPP1_HUMAN

NCBI Description

This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq, Jul 2008]

Uniprot Description

ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.

Protein type: EC 3.1.4.1; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; Membrane protein, integral; Cofactor and Vitamin Metabolism - riboflavin; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 3.6.1.9; Motility/polarity/chemotaxis; Nucleotide Metabolism - purine; Carbohydrate Metabolism - starch and sucrose; Phosphatase (non-protein); Phosphodiesterase

Chromosomal Location of Human Ortholog: 6q22-q23

Cellular Component: extracellular space; cell surface; integral to plasma membrane; lysosomal membrane; basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: protein binding; nucleotide diphosphatase activity; phosphodiesterase I activity; protein homodimerization activity; 3'-phosphoadenosine 5'-phosphosulfate binding; nucleic acid binding; zinc ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; ATP binding; scavenger receptor activity; insulin receptor binding; polysaccharide binding

Biological Process: receptor-mediated endocytosis; generation of precursor metabolites and energy; sequestering of triacylglycerol; vitamin metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; negative regulation of fat cell differentiation; bone remodeling; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; riboflavin metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; regulation of bone mineralization; water-soluble vitamin metabolic process; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process

Disease: Obesity; Cole Disease; Arterial Calcification, Generalized, Of Infancy, 1; Hypophosphatemic Rickets, Autosomal Recessive, 2; Diabetes Mellitus, Noninsulin-dependent

Research Articles on ENPP1

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Product Notes

The ENPP1 enpp1 (Catalog #AAA6002284) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Enpp1, NT (Enpp1, Npps, Pc1, Pdnp1, Ectonucleotide pyrophosphatase/phosphodiesterase family member 1, Lymphocyte antigen 41, Phosphodiesterase I/nucleotide pyrophosphatase 1, Plasma-cell membrane glycoprotein PC-1, Alkaline phosphodiesterase I, Nucleotide reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Enpp1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in Western Blot, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500. Researchers should empirically determine the suitability of the ENPP1 enpp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Enpp1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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