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Western Blot (WB) (Western Blot analysis in Jurkat cell line lysates (35ug/lane), using MBS6002237. This demonstrates that MBS6002237 detected the CLDN22 protein (arrow).)

Rabbit anti-Human, Mouse CLDN22 Polyclonal Antibody | anti-CLDN22 antibody

CLDN22, ID (CLDN22, Claudin-22)

Gene Names
CLDN22; CLDN21
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Flow Cytometry, Functional Assay
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Synonyms
CLDN22; Polyclonal Antibody; ID (CLDN22; Claudin-22); Anti -CLDN22; anti-CLDN22 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Human, mouse
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Applicable Applications for anti-CLDN22 antibody
ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Western Blot, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Flow Cytometry: 1:10-50
Immunogen
CLDN22 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 97-126 amino acids from the Central region of human CLDN22.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Jurkat cell line lysates (35ug/lane), using MBS6002237. This demonstrates that MBS6002237 detected the CLDN22 protein (arrow).)

Western Blot (WB) (Western Blot analysis in Jurkat cell line lysates (35ug/lane), using MBS6002237. This demonstrates that MBS6002237 detected the CLDN22 protein (arrow).)

Western Blot (WB)

(Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane), 039964. This demonstrates that MBS6002237 detected the CLDN22 protein (arrow).)

Western Blot (WB) (Western Blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane), 039964. This demonstrates that MBS6002237 detected the CLDN22 protein (arrow).)

Flow Cytometry (FC/FACS)

(Flow cytometric analysis of Jurkat cells (right histogram), using MBS6002237 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (Flow cytometric analysis of Jurkat cells (right histogram), using MBS6002237 compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-CLDN22 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,509 Da
NCBI Official Full Name
claudin-22
NCBI Official Synonym Full Names
claudin 22
NCBI Official Symbol
CLDN22
NCBI Official Synonym Symbols
CLDN21
NCBI Protein Information
claudin-22
UniProt Protein Name
Claudin-22
Protein Family
UniProt Gene Name
CLDN22
UniProt Entry Name
CLD22_HUMAN

NCBI Description

This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is intronless and overlaps the 3' UTR of the WWC2 gene (GeneID: 80014) on the opposite strand. [provided by RefSeq, Aug 2010]

Uniprot Description

Claudin-22: Plays a major role in tight junction-specific obliteration of the intercellular space, through calcium- independent cell-adhesion activity. Belongs to the claudin family.

Protein type: Membrane protein, multi-pass; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q35.1

Cellular Component: tight junction; integral to membrane; plasma membrane

Molecular Function: identical protein binding; structural molecule activity

Biological Process: intercellular junction assembly and maintenance; calcium-independent cell-cell adhesion

Research Articles on CLDN22

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Product Notes

The CLDN22 cldn22 (Catalog #AAA6002237) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CLDN22, ID (CLDN22, Claudin-22) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CLDN22 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Flow Cytometry (FC/FACS). Suitable for use in Western Blot, Flow Cytometry, ELISA Dilution: ELISA: 1:1,000 Western Blot: 1:100-500 Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the CLDN22 cldn22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CLDN22, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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